. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DEVELOPMENTAL. CELLULAR, AND MOLECULAR BIOLOGY Reference: fiiol. Hull. 193: 233-234. (October. 1997) 233 Comparison of Three Techniques for Evaluating Seasonal Gametogenesis in Spisula solidissima P. Schneider1, R. Smolwit- (Laboratory for Aquatic Animal Medicine and Pathology, University of Pennsylvania, Marine Biological Laboratory, Woods Hole, Massachusetts 02543), C. Smith2, J. Degiorgis1, and M. McCafferty3 solitlissiiiui. the Atlantic surf clam, is widely used in reproductive research. This species is usually
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DEVELOPMENTAL. CELLULAR, AND MOLECULAR BIOLOGY Reference: fiiol. Hull. 193: 233-234. (October. 1997) 233 Comparison of Three Techniques for Evaluating Seasonal Gametogenesis in Spisula solidissima P. Schneider1, R. Smolwit- (Laboratory for Aquatic Animal Medicine and Pathology, University of Pennsylvania, Marine Biological Laboratory, Woods Hole, Massachusetts 02543), C. Smith2, J. Degiorgis1, and M. McCafferty3 solitlissiiiui. the Atlantic surf clam, is widely used in reproductive research. This species is usually considered to produce ripe gametes once during the year in May to June (1. 2, 3). but dual cycles of gametogenesis have been reported in one location (4). Personnel at the Marine Resources Center (MRC) of the Marine Biological Laboratory have noted that 1 MRC, MBL. Woods Hole, MA. : Laboratory of C. Reinisch, Tufts MBL. Woods Hole. MA. 3 LAAMP, U. of MBL. Woods Hole. MA. clams at different sites contain mature gonads at different times of the summer. Inability to predict the gonadal maturity of animals being collected results in wasteful harvest of unripe and partially ripe clams that cannot be used for research. Descriptions of the gross reproductive morphology of the gonad stages is lacking. However, through histologic examina- tion of gonadal tissue sections, five stages have been identified in the reproductive cycle of both sexes of Spisula: early active, late active, ripe, partially spawned, and spent (4). But histologic evaluation of gonadal tissue is time consuming, expensive, and requires sacrifice of the clam. In an effort to identify a quicker. Figure 1. Samples of needle biopsv () and v' morphology (C-E) o/Spicula solidissima gonad: Wet preparation of needle biopsies shows large eggs with well-developed amphinucleoli (arrow) from a ripe female (A) and small eggs from a female ilun is partiully ripe (B). Photographs of gross morphology show a ripe f
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
