. The elements of pathological histology with special reference to practical methods . Fig. 10.—Petris Capsule. According to Esmarclis method, the liquefied gelatin is first of allinoculated in different degrees of dilution, as in the plate is not, however, then poured out, but is diffused over the innerwall of the test-tubes and made to set, by continually turning—rolling—the tubes, which are provided with india-rubber caps, in ahorizontal position in a vessel of iced water or under the stream froma tap, while the other hand steadies the tube.^ When the latter isvery w^ide no gelati
. The elements of pathological histology with special reference to practical methods . Fig. 10.—Petris Capsule. According to Esmarclis method, the liquefied gelatin is first of allinoculated in different degrees of dilution, as in the plate is not, however, then poured out, but is diffused over the innerwall of the test-tubes and made to set, by continually turning—rolling—the tubes, which are provided with india-rubber caps, in ahorizontal position in a vessel of iced water or under the stream froma tap, while the other hand steadies the tube.^ When the latter isvery w^ide no gelatin will penetrate into the plug in rolling, although 1 There is also a special apparatus for rolling several tubes at the sametime by means of a revolving 44 BACTERIOLOGICAL METHODS a partial moistening of the latter would do no harm ; though if thewhole lower surface of the cotton wool should become coated withgelatin, the latter must then be perforated by means of a platinumwire sterilised at a red heat. Esmarchs method has certainly the advantages, as contrasted withthe plate method, of being very expeditious, of being practicable every-where, and of freedom from any danger of contamination whileexamining the colonies. On the other hand, however, the examinationand fishing out of the latter is less convenient, and when amongstthem there are some which liquefy gelatin, the entire culture mayeasily be destroyed. Furthermore, this method cannot be used withagar. Lipezs cult lire-flasks (Fig. 11) are filled and inoculated like thetest-tubes in the plate method, and then simply placed to solidify after Fig. 11.— Culture-Flask. the germs have been evenly distributed by tilting the gelatin or agar—the latter can also be used in t
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectpatholo, bookyear1895
