. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SODIUM PUMPS IN SEA URCHIN EMBRYOS 105 Q- ; fi * E I 1 25 - - 075 - - - 000 -> T. - 100% I* - 80% £ 6 oj E h 60% CD g- M - 40% > 1 - 20% a a - 0% 20 40 60 Age (hours) 80 Figure 4. Total enzyme activity of Na+.K+-ATPase during gastrula- tion in Strongylocentrotus purpuratus. Protein-specific enzyme activity (closed circles) is plotted on the left axis; mRNA levels from Fig. 3 are overlaid (dashed line) to illustrate the temporal relationship between a-sub- unit gene expression and the appearance of
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SODIUM PUMPS IN SEA URCHIN EMBRYOS 105 Q- ; fi * E I 1 25 - - 075 - - - 000 -> T. - 100% I* - 80% £ 6 oj E h 60% CD g- M - 40% > 1 - 20% a a - 0% 20 40 60 Age (hours) 80 Figure 4. Total enzyme activity of Na+.K+-ATPase during gastrula- tion in Strongylocentrotus purpuratus. Protein-specific enzyme activity (closed circles) is plotted on the left axis; mRNA levels from Fig. 3 are overlaid (dashed line) to illustrate the temporal relationship between a-sub- unit gene expression and the appearance of functional sodium pump proteins. Data from Leong and Manahan (1997) are also plotted (open circles) to show the pattern of relative enzyme activities. (, 40% in sea urchin larvae, Leong and Manahan 1997), it would seem to be a good candidate for such an assay, with the potential to provide sensitive information regarding rates of energy utilization in a single larva. Several lines of evidence strongly support the conclusion that the partial cDNA clone (#020) in Strongylocentrotus purpuratus is the a-subunit of the sodium pump: (1) the putative amino acid sequences show a high similarity to those of other animals; (2) the 3'-UTR probe recognizes a transcript, which is the full-length transcript size in other invertebrate species; (3) the ontogenetic increase in expression during gastrulation is similar to the expression pattern in another sea urchin (Mitsunaga-Nakatsubo ct ai, 1992b); (4) total Na+,K+-ATPase enzyme activities show a concomitant increase as mRNA transcripts of clone #020 accumulate during gastrulation. In the sea urchin Hemicentrotus pulcherrimus. the ex- pression of the a-Na+,K+-ATPase gene increases rapidly during gastrulation (Mitsunaga-Nakatsubo et ai, 1992b). In 5. purpuratus, the expression of the a-Na+,K + -ATPase gene evidences a similar pattern of ontogenetic regulation, with a sharp rise during gastrulation followed by a subse- quent declin
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
