. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 404 P. PERSON, A. SMARSH, S. J. LIPSON AND M. R. CARRIKER bored by the snails (see Carriker, Scott and Martin, 1963; and Carriker and Van Zandt, 1964). In view of the fact that cytochrome oxidase is almost always intra- cellular in localization, it is of considerable interest that extracellular oxidase activity should be associated with such secretions. It should be mentioned that the rate of dye synthesis appeared to be more rapid than that customarily seen even with most actively aerobic mammalian tissues studied under sim
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 404 P. PERSON, A. SMARSH, S. J. LIPSON AND M. R. CARRIKER bored by the snails (see Carriker, Scott and Martin, 1963; and Carriker and Van Zandt, 1964). In view of the fact that cytochrome oxidase is almost always intra- cellular in localization, it is of considerable interest that extracellular oxidase activity should be associated with such secretions. It should be mentioned that the rate of dye synthesis appeared to be more rapid than that customarily seen even with most actively aerobic mammalian tissues studied under similar assay conditions. Thus, in the epithelial cells of the ABO, dye formation was quite visible to the unaided eye in 5 minutes, and as seen in Figures 1 and 2 was markedly advanced by 15 minutes. Comparable color intensities in mammalian heart or kidney sections usually require 30 minutes or more of incubation time. It will be seen later that the biochemical data support this impression of greater activity in the ABO epithelial tissue. b. Succinate dehydrogenase. When malonate, a classical inhibitor of succinic dehydrogenase, was made equimolar to succinate in the incubation mixture ( 10~2 M), it strongly inhibited activity. Omission of succinate from the reaction mixture resulted in complete absence of color formation in the interval studied (, 3-10 minutes). Figure 3 shows that the distribution of succinate dehydrogenase activity almost completely paralleled that of cytochrome oxidase, with the possible exception SC. SE- FIGURE 3. (Left) Distribution of histochemical succinate dehydrogenase in a saggital freshly frozen section of Urosalpinx ABO. Activity appears in locations similar to that seen for cytochrome oxidase in Figures 1 and 2; only secretory epithelial cells (SC) show activity, while gland stroma (GS) and stalk epithelium (SE) are negative. Externally secreted material (ES) at the outer surface of the secretory cells shows a positive reaction. Magnifica-
Size: 2012px × 1242px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
