. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 0 16 20 TIME (Days) £ ii 0. U Q -S" cu U. TIME (Days) Figure 11. '4C-Glucose tracer content of tissues during early disc regeneration. (A) Natural seawater experiment. (B) Artificial seawater experiment. AC = Artificial seawater control, AR = Artificial seawater regenerating, NC = Natural seawater control. NR = Natural seawater regenerating, DIST = Distal arm, PROX = proximal arm and oral frame. Error bars represent 95% confidence intervals. Error bars and points offset slightly for graphical clarity. all non-regenerati
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 0 16 20 TIME (Days) £ ii 0. U Q -S" cu U. TIME (Days) Figure 11. '4C-Glucose tracer content of tissues during early disc regeneration. (A) Natural seawater experiment. (B) Artificial seawater experiment. AC = Artificial seawater control, AR = Artificial seawater regenerating, NC = Natural seawater control. NR = Natural seawater regenerating, DIST = Distal arm, PROX = proximal arm and oral frame. Error bars represent 95% confidence intervals. Error bars and points offset slightly for graphical clarity. all non-regenerating body parts, but the overall trend was similar to that exhibited by the artificial seawater control group in that most of the loss was from the oral frame and arm tips. The subsequent increase in organic material appeared to be localized in the arms. The relative protein content of the body increased constantly during regen- eration, indicating either a net gain of protein during re- generation, or a loss of minerals as tissue breakdown oc- curred. This gain could be due to a net uptake of proteins (or amino acids) from the medium, or a combination of uptake and overall loss of other body biochemical con- stituents during regeneration. Although the carbohydrate and lipid content of the body did not change significantly over the same period, the latter explanation is more likely, because the animals lost total caloric content constantly during regeneration. The increase in organic material in the non-regenerating portions of the body after day 12 is problematical, because no corresponding increase in bio- chemical constituents in those parts could be demon- strated. This increase might be explained as the summa- tion of non-significant increases in each biochemical con- stituent to make a significant increase in total organics. Animals regenerating in the absence of exogenous nu- trients constantly lost organic material from non-regen- erating body parts. The rate of loss was
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
