. Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 . , Fig. I. Double reciprocal plots of interference by K {left) and Na with Rb aljsorption by excised barley roots. Rb concentration, S, 2 to 10 mEq/1. Rate of Rb absorption, v, in nig/gm fresh weight in 3 hr. out a change in the slope. The reader is referred to the paper by Wilson (45) for a classification of the various types of inhibition. To test the validity of the enzyme-kinetic hypothesis of the ope
. Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 . , Fig. I. Double reciprocal plots of interference by K {left) and Na with Rb aljsorption by excised barley roots. Rb concentration, S, 2 to 10 mEq/1. Rate of Rb absorption, v, in nig/gm fresh weight in 3 hr. out a change in the slope. The reader is referred to the paper by Wilson (45) for a classification of the various types of inhibition. To test the validity of the enzyme-kinetic hypothesis of the operation of carriers we measured the rate of ion absorption by excised barley roots as a function of the concentration of the ion being absorbed (the 'substrate ion') and the effect of other ions of the same sign present in the solution ('interfering ior^s'). Figure i presents the results of two typical experiments on Rb absorp- tion. The three features of interest are these: i) the absorption rate varies in response to the Rb concentration in the manner expected; straight lines are obtained when the reciprocal of the rate of absorption is plotted against the reciprocal of the substrate (Rb) concentration, according to Lineweaver and
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Keywords: ., bookcentury1900, booksubjectelectrolytes, booksubjectphysiologyc
