. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 3iS R. BERMAN, I. B. WILSON, D. NACHMANSOHN VOL. 12 (1953) assay based upon the glutathione extinction coefficient and the biological acetylcholine measurement; Time (min) fiM of acetylcholine formed jml Bioassay Nitroprusside test 30 60 0-33 0-34 These data validate the appearance of mercaptan as a measure of acetylation. However, it must be noted that there is a difference - not yet explained - between the two systems: In the full sys
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 3iS R. BERMAN, I. B. WILSON, D. NACHMANSOHN VOL. 12 (1953) assay based upon the glutathione extinction coefficient and the biological acetylcholine measurement; Time (min) fiM of acetylcholine formed jml Bioassay Nitroprusside test 30 60 0-33 0-34 These data validate the appearance of mercaptan as a measure of acetylation. However, it must be noted that there is a difference - not yet explained - between the two systems: In the full system including transacetylase, acetylphosphate etc. the activity was about 3 times as high as in the tests with choline acetylase alone. RESULTS Figs. I and 2 demonstrate the time course of the acetylation reaction for different concentrations of the substrates choHne and acetyl Co A. The choline curves show very 10 15 20 40 Choline u. mol/ml iMg. I. Acetylation of choline by choline ace- Ivlase as function of choline concentration. The reaction mixture contained 140 jug en- zyme and the following components in juM per ml: Acetyl CoA 5, phosphate buffer pH 7, yo, choline as indicated in the graph. Total volume I ml, t = 3i°C. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Burk, Dean. Amsterdam, Houston, Elsevier Pub. Co.
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