. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 440 H. SUGITA AND K. SEKIGUCHI supernatant bands on a gel. Therefore, we used Davis' (1964) buffer system to examine the developing MDH patterns in Asian horseshoe crabs and their hybrids. There were electrophoretic variants of the MDH in 3 Asian horseshoe crabs, but genetic variants of the MDH were not detected in larvae developed from eggs of a single female (compare the MDH-1 of columns Tg, gt, and gr in Fig. 3 with that of columns Tg and gt in Fig. 4). The early embryos displayed only the maternal forms of both MDH-1 and
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 440 H. SUGITA AND K. SEKIGUCHI supernatant bands on a gel. Therefore, we used Davis' (1964) buffer system to examine the developing MDH patterns in Asian horseshoe crabs and their hybrids. There were electrophoretic variants of the MDH in 3 Asian horseshoe crabs, but genetic variants of the MDH were not detected in larvae developed from eggs of a single female (compare the MDH-1 of columns Tg, gt, and gr in Fig. 3 with that of columns Tg and gt in Fig. 4). The early embryos displayed only the maternal forms of both MDH-1 and MDH-2 in hybrid and normal progeny and these enzyme forms were detected in unfertilized eggs of 3 species (Fig. 3). The paternal form of the MDH-1 was first detected in the T. tridentatus 9 X C. rotundicauda 6 embryo at stage 14 (stage of appearance of rudimental appendages, 28 days after insemination) (Fig. 4A), while in the hybrid embryo of the reciprocal cross the paternal forms of the MDH-1 and MDH-2 were not expressed even on the 41st day after insemination (stage 19, after the 2nd embryonic molt). The paternal form of the MDH-2 was displayed in the T. gigas 9 X T. tridentatus <3 embryos at stage 19 (after the 2nd embryonic molt) and stage 20 (after the 3rd embryonic molt) (Fig. 4B). This paternal form in the T. gigas 9 X T. tridentatus <3 embryo was first observed at stage 17 (22 days after insemination, immediately before the 1st embryonic molt, results not shown). On the other hand, the hybrid embryos whose development was stopped at blastula stage, that is, T. tridentatus 9 X T. gigas <3, T. gigas 9 X C. rotundicauda B MDH-1 MDH-2 I. tr rt Cr Cr rg gr Tg Tg gt tg Tt Ti 155U53 U 666 6U FIGURE 3. Electrophoretic patterns of the MDH in unfertilized eggs and early embryos of 3 Asian horseshoe crabs and their hybrids. The 3rd-day and 6th-day embryos from a hybridization experiment were used in A and B, respectively. Horizontal starch gel electrophoresis was carrie
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
