. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GROWTH AND ACTION OF EYE-STALK EXTRACT 253 For a preliminary trial young seedlings of Lupin us from which the root tip had been removed were " seated " on the smooth edge of small vials containing 2 ml. of extract. The decapitated root was immersed hv about 5 mm. in the extract. Reactions were of the same order as J with the agar blocks. In Fig. 1 curves ./ and /-> show the elongation of two decapitated Arena coleoptiles which had been capped with blocks of agar imbibed with freshly prepared eye-stalk extract. T
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GROWTH AND ACTION OF EYE-STALK EXTRACT 253 For a preliminary trial young seedlings of Lupin us from which the root tip had been removed were " seated " on the smooth edge of small vials containing 2 ml. of extract. The decapitated root was immersed hv about 5 mm. in the extract. Reactions were of the same order as J with the agar blocks. In Fig. 1 curves ./ and /-> show the elongation of two decapitated Arena coleoptiles which had been capped with blocks of agar imbibed with freshly prepared eye-stalk extract. The increase in rate becomes apparent within 15 minutes of the time the block is placed on the cut surface, and bears every similarity to the response of the decapitated coleoptile to the growth hormone itself. It is important to consider that the extract from the eye-stalk con- tains NaCl and probably numerous other substances. Control observa- tions were made with agar blocks imbibed with salt solution of the same X h- o z. I I I I I I TIME (I UNIT = 60MIN.) FIG. 2. Curves A and B show effect of Palcemonetcs abdominal muscle ex- tract upon elongation of Avena coleoptiles. Curves C and D show the effect of blocks of thoroughly leached agar without eye-stalk extract upon the elongation of Arena coleoptiles. concentration as that used in preparing the experimental extracts, and with extract of the abdominal musculature of light-adapted Palcemo- tietcs. The extract of abdominal muscle was made by first washing in distilled water 100 abdomens, then macerating them without the addi- tion of fluid. One milliliter of distilled water was then added to it with a few crystals of salt, the mass boiled and centrifuged, the supernatant liquid decanted and made up to 2 ml. Each ml. of the extract thus corresponded to one abdomen. Injections of ml. of the extract of abdominal muscle were made into both white-adapted and black-adapted Palccmonetcs, and were found to be wholly without
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
