Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . directlythrough, so that it appears on the other side, then the foldlet go, the needle withdrawn just far enough so as to be withinthe cavity. Inoculation in the Eye.—The anterior chamber and thecornea are the two places used. The rabbit is fixed upon aboard, the eyelids held apart and head held still by an assist-ant. A few drops of cocain having first been introduced inthe eye, a small cut is made in the cornea. The material ispassed through the opening with a small f
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . directlythrough, so that it appears on the other side, then the foldlet go, the needle withdrawn just far enough so as to be withinthe cavity. Inoculation in the Eye.—The anterior chamber and thecornea are the two places used. The rabbit is fixed upon aboard, the eyelids held apart and head held still by an assist-ant. A few drops of cocain having first been introduced inthe eye, a small cut is made in the cornea. The material ispassed through the opening with a small forceps, and with afew strokes of a spoon it is pushed in the anterior chamber. ANIMAL INOCULATION 93 For the cornea a few scratches made in the corneal tissuewill suiSce; the material is then gently rubbed in. Inoculation of the Cerebral Membranes.—^The skinand aponeurosis cut through where the skull is the the bone carefully trephined, and the dura exposed. Inrabies inoculation, the syringe containing the hydrophobicvirus pierces the dura and arachnoid, and the virus is dis-charged beneath the Fig. 37.—Method of making an intravenous injection into a that the needle enters the posterior vein from the hairy sur-face. Intratracheal.—The bacteria can be introduced directlyinto the trachea, thus coming in contact with the lungs. Intraduodenal.—Cholera germs are injected into the in-testines after they have been exposed by carefully openingthe abdomen. This is done in order to avoid the action ofthe gastric juice. Celloidin sacs of small size are sometimes used to intro- 94 ESSENTIALS OF BACTERIOLOGY duce living cultures of bacteria into the bodies of animalswithout their coming into direct contact with the tissues. Obtaining Material from Infected Animals.—^The ani-mal should be skinned, or the hairs plucked out, before it iswashed—at least the portion where the incision is to be the entire body is washed in sublimate.
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Keywords: ., bookcentury1900, bookdecade1910, bookpublisherphila, bookyear1913
