. Essentials of laboratory diagnosis; designed for students and practitioners. will ferment,giving off bubbles of C02, and at the same time formingalcohol. By measuring the amount of carbon dioxid gas formedduring this fermentation process, we are enabled to estimate thepercentage and amount of sugar contained in the specimen un-der examination. A specially shaped and graduated tube (seeFig. 46) is usually employed in this test. It is known as theEinhorn saccharimeter. The upright tube is graduated from itsclosed upper end downward from to 1 per cent. It will notestimate sugar in amounts l
. Essentials of laboratory diagnosis; designed for students and practitioners. will ferment,giving off bubbles of C02, and at the same time formingalcohol. By measuring the amount of carbon dioxid gas formedduring this fermentation process, we are enabled to estimate thepercentage and amount of sugar contained in the specimen un-der examination. A specially shaped and graduated tube (seeFig. 46) is usually employed in this test. It is known as theEinhorn saccharimeter. The upright tube is graduated from itsclosed upper end downward from to 1 per cent. It will notestimate sugar in amounts less than per cent., and owing tothe varying solubility of the various gases of the urine, depend-ing on differing reactions and the density, it cannot be regardedas absolutely accurate. FERMENTATION SACCHARIMETER. 275 The Test.—Urines containing less than 5 per cent, ofglucose must be diluted five times with water. Urines contain-ing more than 5 per cent, must be diluted ten times before pro-ceeding with the test. Mix in a large test-tube about 20 cubic centimeters of. A B Fig. 46.—Einhorn Saccharimeter in Use. A shows Gas formed after Twenty-four Hours from a Diabetic Urine (Dilution 1 to 10) containing Per Cent, of Sugar; B, Control with Normal Urine. properly diluted urine with one-twelfth of a cake of freshcompressed yeast. Completely fill the upright tube of thesaccharimeter with this mixture, so as to exclude all air fromthe graduated tube. Fill a second saccharimeter with yeastdissolved in distilled water or with urine known to be free fromsugar. These tubes are to be kept in a warm place andallowed to remain undisturbed for from eighteen to twenty- 276 THE URINE. four hours. Br this time the sugar-containing urine will befound to have been displaced by gas in the vertical tube. Thepercentage corresponding to the level of the fluid will representthe percentage of sugar in the diluted urine. This figure, mul-tiplied by the dilution, will represent the percenta
Size: 1486px × 1681px
Photo credit: © Reading Room 2020 / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1910, bookpublisheretcetc, bookyear191
