. Pathological technique; a practical manual for workers in pathological histology and bacteriology. sed carbon-dioxid, which is found in commerce in iron cylinders con-taining each about twenty pounds of liquefied gas. It iscommonly used for charging beer and soda-water. As arule, the cylinders are loaned, so that it is necessary to payfor the contents only. The cylinder must be securely fastened in an uprightposition near the microtome, with its valve end below andwith its escape-tube on a level with the entrance-tube intothe freezing-box. The cap covering the escape-tube of thecylinder shou
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. sed carbon-dioxid, which is found in commerce in iron cylinders con-taining each about twenty pounds of liquefied gas. It iscommonly used for charging beer and soda-water. As arule, the cylinders are loaned, so that it is necessary to payfor the contents only. The cylinder must be securely fastened in an uprightposition near the microtome, with its valve end below andwith its escape-tube on a level with the entrance-tube intothe freezing-box. The cap covering the escape-tube of thecylinder should have a small hole bored through it, and intothis hole a small brass tube about 5 cm. long, with a finebore, should be tightly driven. This permits the use of a HISTOLOGICAL METHODS. 21 smaller stream of gas than the escape-pipe of the cylinderwould otherwise furnish. The same cap can be kept to useon all future cylinders. The cylinder is connected with the microtome by meansof a short piece of thick, strong rubber-tubing with smallbore, so as to fit snugly over the escape-tube of the cylinder. Fig. i.—Freezing microtome. and the entrance-tube into the freezing-box. It is advisableto wire each end of the rubber-tubing around the tube itincloses. The connection can also be made with flexiblecopper tubing. In order to obtain better leverage and more perfect con-trol over the escape of the gas than are needed for thepurposes for which the cylinders are ordinarily used, it isnecessary to lengthen to about 25 cm., in whatever way 22 PATHOLOGICAL TECHNIQUE. seems best, the handle of the key which opens the escape-valve. The first time the cylinder is used for freezing, a littlewater may escape, causing considerable sputtering. Infreezing, the valve should be turned carefully, so that the gasmay escape slowly and evenly. Tissues fixed by alcohol orany other reagent, except formaldehyde, must be washed inrunning water for some hours before they can be frozen. Even for tissue fixed
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