. The diagnosis of diseases of women . cision in opening the uterus after hysterectomy. 1. Stain in hsematoxylin one to two minutes. 2. Decolorize in acid alcohol. 3. Immerse in weak ammonium-water until the blue colorreturns. 4. Immerse in water to remove the ammonium. 5. Counterstain in eosin from ten to thirty seconds. 6. Immerse in 75 per cent, alcohol two minutes. 7. Absolute alcohol one minute. - .. 8. Clear in creosote or oil of cloves. 9. Mount in Canada balsam. , 100 GENERAL DIAGNOSIS Paraffin Sections. After cutting the sections they are carefullytransferred to a shallow basin o
. The diagnosis of diseases of women . cision in opening the uterus after hysterectomy. 1. Stain in hsematoxylin one to two minutes. 2. Decolorize in acid alcohol. 3. Immerse in weak ammonium-water until the blue colorreturns. 4. Immerse in water to remove the ammonium. 5. Counterstain in eosin from ten to thirty seconds. 6. Immerse in 75 per cent, alcohol two minutes. 7. Absolute alcohol one minute. - .. 8. Clear in creosote or oil of cloves. 9. Mount in Canada balsam. , 100 GENERAL DIAGNOSIS Paraffin Sections. After cutting the sections they are carefullytransferred to a shallow basin of warm water, on which they spreadin thin ribbons. The water must not be hot enough to melt theparaffin, but merely sufficiently so to unfold the sections and spreadthem out smoothly. A glass slide is held underneath the sections,and they are made to float upon the slide. The slide is then with-drawn from the water, the water drained off, and is then placed forseveral hours on the top of an oven or radiator, where the moisture Fig. 24. The uterine cavity exposed. is thoroughly driven from the slide and the section firmly paraffin is dissolved in zylol or chloroform (by which the sectionis ^cleared), and from this point on the staining is carried out inthe usual manner. *■ INSPECTION OF THE UTERUS AFTER REMOVAL. In order that a satisfactory examination rnay be made of theuterus after its removal, the operator should handle and mutilate thespecimen as little as possible. Introduction of swabs, probes, and MICROSCOPIC EXAMINATION OF SCRAPINGS IQl curettes injure the endometrium and lead to false 23 shows the method of opening the uterus. The body of theuterus is grasped by the left hand. Two incisions are made, asshown in Fig. 24, and the uterus is spread open in such a mannerthat the entire mucosa*will be exposed. Before the uterus is openedit is always well to fix it in Zenkers fluid for several days. Thestructures are thereby least disturbed in the
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