. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CELL BIOLOGY 203 Reference: Hiol. Hull 203-204. (October 20(12) Immunohistochemical Demonstration of a Lipopolysaccharide in the Cell Wall of a Eukaryote, the Green Alga, Chlorella Peter B. AnnMroni> (Department of Molecular and Cellular Biology, University of California, Davis. California), Margaret T. Armstrong1, R. L Partly2, Alice Chili?, ami Norman Wainwright* The lipopolysaccharides (LPS) are ubiquitous components of the outer leaflet of the outer membrane of all gram-negative bacteria and are the principal tox
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CELL BIOLOGY 203 Reference: Hiol. Hull 203-204. (October 20(12) Immunohistochemical Demonstration of a Lipopolysaccharide in the Cell Wall of a Eukaryote, the Green Alga, Chlorella Peter B. AnnMroni> (Department of Molecular and Cellular Biology, University of California, Davis. California), Margaret T. Armstrong1, R. L Partly2, Alice Chili?, ami Norman Wainwright* The lipopolysaccharides (LPS) are ubiquitous components of the outer leaflet of the outer membrane of all gram-negative bacteria and are the principal toxic products of these organisms (I). The membrane anchor of LPS is lipid A, a central phosphodisac- charide unit that is attached to multiple jB-hydroxy fatty acid chains. LPS also contains the novel sugar. 3-deoxy-D-manno- octulosonic acid (KDO). Although generally believed to be re- stricted to prokaryotes. specifically the gram-negative eubaeteria and the cyanobacteria (2). an LPS-like molecule has recently been reported from a eukaryote. the green alga Chlorella strain NC64A. The algal molecule includes KDO, lipid A. and /3-hy- droxy fatty acids and is thus chemically similar to bacterial LPS ' Department of Molecular and Cellular Biology. University of Califor- nia, Davis. CA 95616. - School of Biological Sciences. University of Nebraska. Lincoln, NE 68583. 1 Marine Biological Laboratory, Woods Hole, MA 02543. (31. The subject of this study is the localization of the LPS-like molecule in the algal cell. One of the defenses raised against LPS by the horseshoe crab. Liinnliis, is a small. 101-amino acid, cationic protein—Liinulus anti-LPS factor (LALF) (4)—which is released from the secretory granules of the blood cells during their exocytosis response to LPS challenge (5). LALF binds and neutralizes bacterial LPS (6). In the present study, we use the specific LPS-binding activity of LALF to localize the LPS-like molecule in eukaryotic and prokaryote cells. The react
Size: 1896px × 1318px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
