Electron microscopy; proceedings of the Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . electronmicrosco00euro Year: 1957 //- Fig. 1. /?-cytomembranes in the non-pigmented epithelium of the cihary body. Magnification 70,000. that the central, osmiophobic layer, because of its regular thickness, is not a simple extracellular tissue space. The membranes are localized only to the apical parts of the cell, and do not extend deeper into the cell than to the apical part of the nucleus. Outside the cell membrane a basement membrane is observed 250-350 A thick, separat
Electron microscopy; proceedings of the Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . electronmicrosco00euro Year: 1957 //- Fig. 1. /?-cytomembranes in the non-pigmented epithelium of the cihary body. Magnification 70,000. that the central, osmiophobic layer, because of its regular thickness, is not a simple extracellular tissue space. The membranes are localized only to the apical parts of the cell, and do not extend deeper into the cell than to the apical part of the nucleus. Outside the cell membrane a basement membrane is observed 250-350 A thick, separated from the cell membrane by an interspace 400-700 A wide. The mitochondria are relatively small with a mean width of only //. Most of them are built up in the common way, with the inner double membranes oriented mainly perpendicularly to the outer double membrane. In some cases, however, the inner mem- branes extend parallel to the long axis of the mito- chondrion and along its whole length. In none of these cases have any connections between the inner and outer membranes been seen. The Golgi apparatus (fig. 2) is without exception encountered in the basal part of the cells. In each cell one can observe 1-3 Golgi units, each consisting of the well-known components: 2-3 rows of double membranes, 7-cytomembranes, a few larger vacuoles, and several small vesicles. Each single osmiophilic component of the double membranes is about 50 A thick. Both the vacuoles and the vesicles are bounded by a single membrane, about 50 A thick. Changes in the idtrastructure after injection of Diamox.—Diamox produces changes in the non- pigmented epithelium in at least three of its compo- nents: the cytoplasm, the mitochondria and the Golgi apparatus. The most marked changes in the cytoplasm is seen within 30 minutes after injection of Diamox. The cells are filled with small vesicles, in the deeper layers mostly very regularly spread, but close to the surface often congregated in large group
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