. The actinomycetes. Actinomycetales. M()|;i'ii()1,();;n , ('^'l'()l,(»(;^, and : ('^?('IJ•:s 77. FlGiRE 37. Sporuliition of .S. r///.s('//.s (Rpprodufed from: Haldacci, K. and (Irciii, A. (Jiorn. Micro- biol. 1: 34. 1955). crosc'ope, the edge of the colony shows a characteristic picture of radiating hyphae. Wlien grown in liiiuid niecUa in a stationary condition, the colonies may be formed in- dividually on the bottom of the container, or they may adhere to the surface of the wall, or they may form a ring of growth or a pelli- cle on the surface. The colonies may also grow in the form o


. The actinomycetes. Actinomycetales. M()|;i'ii()1,();;n , ('^'l'()l,(»(;^, and : ('^?('IJ•:s 77. FlGiRE 37. Sporuliition of .S. r///.s('//.s (Rpprodufed from: Haldacci, K. and (Irciii, A. (Jiorn. Micro- biol. 1: 34. 1955). crosc'ope, the edge of the colony shows a characteristic picture of radiating hyphae. Wlien grown in liiiuid niecUa in a stationary condition, the colonies may be formed in- dividually on the bottom of the container, or they may adhere to the surface of the wall, or they may form a ring of growth or a pelli- cle on the surface. The colonies may also grow in the form of flakes, but the medium is never made turbid, as in the case of l)ac- tei'ial growth, unless the colonies undergo lysis through the action of enzymes or phages. When grown in a submerged or in a .shaken condition, actinomycetes produce small, bead-like masses of growth, some of which may l)e granular in natui'e. According to , strains of Xoranlia with more persistent mycelium produce firm agar colonies and a discretely graiuilar growth in licjuid media, which remain clear; those with small or rapidly dixiding myce- lium show a soft growth in solid media and a diffuse, bacterium-like growth in liquid media. Jensen took exception to Lieske's (1921) .statement that "every form of tur- bidity of a li{[uid medium is to be looked upon as evidence of ; He also pointed out that a similar difference exists in cultures of the genus Actinomyces, where most strains show a granular growth V)ut some gi\'e a clcjudy growth in broth (Erik- son, 1940; Holm, 1948). The colonies of the bovine strains of this anaerobic genus Actino- mijcc's are smooth and soft in consistency and do not adhere to the medium; the mycelium undergoes fragmentation very rapidly, giv- ing no extensive ramification; .such strains show occasional turl)idity in the medium. In contrast, hun;an strains give no stable variants and produce no turbidity (). Various attempts have been m


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