. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 250 D. J. QUINN AND C. E. LANE axon (Caldwell and Keynes, 1959). Ouabain specifically inhibits the NaKATPase activity isolated from red blood cells (Post ct «/., 1960; Dunham and Glynn, 1961 ), crab nerve (Skou, 1957), gill tissue homogenates of C. giianhuini (Quinn and Lane, 1966), and other tissues (Bonting ct al., 1961 ; Whittam, 1962; Glynn, 1963). Brain mitochondria] respiration measured in the presence of brain microsomes and ouabain is reduced by about 4Qr/r, due presumably to the inhibition by ouabain of the NaKATPas
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 250 D. J. QUINN AND C. E. LANE axon (Caldwell and Keynes, 1959). Ouabain specifically inhibits the NaKATPase activity isolated from red blood cells (Post ct «/., 1960; Dunham and Glynn, 1961 ), crab nerve (Skou, 1957), gill tissue homogenates of C. giianhuini (Quinn and Lane, 1966), and other tissues (Bonting ct al., 1961 ; Whittam, 1962; Glynn, 1963). Brain mitochondria] respiration measured in the presence of brain microsomes and ouabain is reduced by about 4Qr/r, due presumably to the inhibition by ouabain of the NaKATPase activity contained in the microsomes (Whittam and Blond, 1964). O ^ I- ^ Q. ~ r ,- -o O UJ O e> — >- =*- X O O - NoCl+KCI A - CHOLINE CHLORIDE. 0 0 0 120 240 360 480 mM NaCI mM KCI '23 245 368 490 mM CHOLINE CHLORIDE FIGURE 5. Effect of NaCI + KCI, and choline chloride on excised gill respiration. Pro- cedure is the same as for previous figures, except that NaCI and KCI were added together. Each point is an average derived from 18 different crabs. In addition, the fraction of the total oxygen consumption which is stimulated by Na+ + K+ in rat brain homogenates (Samson and Quinn, 1967) and in the gall bladder of the rabbit (Martin and Diamond, 1966) is inhibited by ouabain, in- dicating that a large percentage of respiratory energy flows through the Na- KATPase systems in these tissues. Similarly, the Q02 of excised gills of C. guanhumi recorded in 75% sea water was reduced by low concentrations of ouabain (Fig. 2) to less than the level attained by osmotic disruption of the tissue in 175% sea water (Fig. 1).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ).
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
