The Journal of biological chemistry . standard solution of intermediateconcentration. The theoretical concentration of this standardwas mg. per 10 cc. The known amounts of phenol in 10 the other solutions and the amounts found by comparisonagainst the standard were as shown in Table II. N. W. Rakestraw 113 Fig. 1 shows the results of plotting the amounts foundagainst the amounts present. The straight line is the idealcondition representing perfect proportionality. The crossesrepresent the actual results. It can be seen that the amount ofphenol may safely be as little as half, or a
The Journal of biological chemistry . standard solution of intermediateconcentration. The theoretical concentration of this standardwas mg. per 10 cc. The known amounts of phenol in 10 the other solutions and the amounts found by comparisonagainst the standard were as shown in Table II. N. W. Rakestraw 113 Fig. 1 shows the results of plotting the amounts foundagainst the amounts present. The straight line is the idealcondition representing perfect proportionality. The crossesrepresent the actual results. It can be seen that the amount ofphenol may safely be as little as half, or as much as twice, the TABLE II. Ratio. Ratio. Present. Found. Foun-l:l^ Present. Found. Found:present. mg. mg. m:i. m:/. (Standard.) Each result is the average of at least three closely nq. Fig. 1. Ratio between color intensity and concentration of phenol. amount in the standard and still retain satisfactory proportionalitybetween color intensity and concentration. Separation of Uric Acid and Phenol. Pelkan seems to have obtained unsatisfactory results in theseparation of uric acid and phenol by precipitation with zinc 114 Determination of Phenols in Blood chloride and sodium carbonate. The present \\Titer has experi-enced no such difficulty. Not only was complete separationobtained, but no traces of zinc could be found in the filtrate. To 25 cc. of solutions containing both uric acid and phenol wereadded 1 cc. of per cent zinc chloride and, after mixing, 1 cc. of10 per sodium carbonate. The precipitate was allowed to floccu- TABLE III. Uric acid. Phenol. Time between Present. Found. Recov-ered. Present. Found. Recov-ered. precipitation andcentrifuging. mg.
Size: 2370px × 1055px
Photo credit: © The Reading Room / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1900, booksubjectbiochem, bookyear1905
