Electron microscopy; proceedings of the Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . electronmicrosco00euro Year: 1957 Fig. 4. Electron miciograpli of a small part of the highly differentiated Golgi zone of a neurone. The line A-B in fig. 1 passes throughasimilar region. Magnification 21,000. of the cell body lying next to the nucleus. In larger neurones they extend from the nucleus (which is excentrically placed) into much of the cell body. Additional details of the structure of the Golgi filaments are revealed when cells are treated by Kolatchev's method a
Electron microscopy; proceedings of the Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . electronmicrosco00euro Year: 1957 Fig. 4. Electron miciograpli of a small part of the highly differentiated Golgi zone of a neurone. The line A-B in fig. 1 passes throughasimilar region. Magnification 21,000. of the cell body lying next to the nucleus. In larger neurones they extend from the nucleus (which is excentrically placed) into much of the cell body. Additional details of the structure of the Golgi filaments are revealed when cells are treated by Kolatchev's method and examined by electron mi- croscopy. The Golgi apparatus is found to consist of chromophilic and chromophobic components as in vertebrate cells (3, 6). Examination of neurones fixed in buffered osmic acid (7) shows that the Golgi filaments are located in a highly diflFerentiated zone of the cell body (fig. 4). The filaments consist of paired anastomosing mem- branes which enclose two substances; a dense material lying within narrow folds of the membranes, and an osmiophobic substance lying within small dilations of the membranes (fig. 3). Lying next to the filaments and scattered about much of the differentiated zone are numerous small (Golgi) vesicles. Some discrete Golgi vacuoles are also present. Comparison of material treated by Kolatchev's method with that fixed in buff'ered osmic acid shows that the chromophilic component of the Golgi apparatus corresponds to the membranes together with the dense substance they enclose. The chromo- phobic component of the Golgi apparatus corre- sponds to the osmiophobic substance lying within dilations of the membrane. Finally the weakly osmiophilic substance (seen in Kolatchev's prepara- tions examined by light microscopy) arises from the impregnation of the Golgi vesicles. The weakly osmiophilic substance is probably the 'archoplasm' of previous workers (2). Filaments are not seen in very small neurones fixed in buffered osmic acid. Ho
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