. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AMPHIBIAN YOLK PLATELET PROTEINS 287 CO .26 .24 .22 .20 .18 .16 LJ O .14 _J .12 |,o S .08 .06 .04 .02. 400 420 440 460 480 500 WAVELENGTH (M|J) 520 540 FIGURE 2. Absorption spectrum of yolk xanthophylls in petroleum ether. Absorption maximum at 440 m/it. pigment was preferentially soluble in 90% methanol with only a small portion remaining in the petroleum ether epiphase. Photometric analysis of the methanol hypophase carotenoids redissolved in petroleum ether showed a light absorption maximum near 440 m//. (see Figure 2). i
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AMPHIBIAN YOLK PLATELET PROTEINS 287 CO .26 .24 .22 .20 .18 .16 LJ O .14 _J .12 |,o S .08 .06 .04 .02. 400 420 440 460 480 500 WAVELENGTH (M|J) 520 540 FIGURE 2. Absorption spectrum of yolk xanthophylls in petroleum ether. Absorption maximum at 440 m/it. pigment was preferentially soluble in 90% methanol with only a small portion remaining in the petroleum ether epiphase. Photometric analysis of the methanol hypophase carotenoids redissolved in petroleum ether showed a light absorption maximum near 440 m//. (see Figure 2). iixcept for a small gel-like fraction (presumably denatured yolk protein) which collected at the saline-ethanol: ether interface, most of the lipid-extracted YPna material (YPdf) remained soluble in lytic concentrations of NaCl. YPdf retained the solubility properties of YPna and, upon dilution, could be precipitated as YP- or YC-like precipitates at the proper salt concentrations. However, the YC-like precipitate in M NaCl, although dense and viscous, was not yellow and trans- parent like normal YC. Also, it was easily scraped or poured from dialysis tubing, whereas YC was very sticky. 3. Electrophoresis As indicated in Figure 3, all the yolk fractions tested exhibited two main electrophoretic peaks: a smaller, faster component (A) and a larger, slower com- ponent (B). The descending patterns showed in addition a variable number of minor peaks which may represent additional electrophoretic species. The trailing margin of the larger B component of YPdf showed some asymmetry, which suggests. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore,
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