. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 154 IGOR TAMM Lee virus • Virus aErythrocytes MB virus •Virus °Irythrocytes. Win. 20 40 60 80 20 40 60 80 100 120 140 Adsorption I Elution FIGURE 1. Relation of adsorption and elution of virus to agglutination and disagglutination of erythrocytes. With both Lee and MB viruses, adsorption was carried out at 4° C. Elution was permitted to take place at 14° C. with Lee virus, and at 37° C. with MB virus. In both experi- ments the control virus titer was 1024. Chicken erythrocytes, final concentration 5 per cent, were used.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 154 IGOR TAMM Lee virus • Virus aErythrocytes MB virus •Virus °Irythrocytes. Win. 20 40 60 80 20 40 60 80 100 120 140 Adsorption I Elution FIGURE 1. Relation of adsorption and elution of virus to agglutination and disagglutination of erythrocytes. With both Lee and MB viruses, adsorption was carried out at 4° C. Elution was permitted to take place at 14° C. with Lee virus, and at 37° C. with MB virus. In both experi- ments the control virus titer was 1024. Chicken erythrocytes, final concentration 5 per cent, were used. , employing MB virus, longer periods were required with tautog, sea robin and dogfish erythrocytes than with mackerel or turtle cells. In no case was evidence of disagglutination observed when tests were done at 4° C. It appeared, therefore, that disagglutination wras a regular phenomenon and that the exceptional instances where disagglutination was not observed may have been due to experimental conditions used: if temperatures higher than 25° C. and periods longer than 4 hours had been employed, disagglutination might have occurred in such instances. DISCUSSION The finding that influenza, mumps and Newcastle disease viruses are capable of agglutinating erythrocytes from teleosts and elasmobranchii is of interest because it focuses attention on the extremely broad species range of this reaction : the agglutina- tion of reptilian, amphibian, avian and mammalian erythrocytes by representatives of this group of viruses has been demonstrated previously (Smadel, 1948). Studies with chicken erythrocytes have yielded suggestive evidence that in the hemagglutina- tion reaction influenza, mumps and Newcastle disease viruses combine with receptor groups on erythrocytes which are mucoprotein in nature (de Burgh et al., 1948; Hirst, 1948). Strong, although indirect, support for this contention has come from studies with mucoid components of tissues and body fluids which react wit
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