. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Cultivation 697 this bacillus upon artificially prepared media, but in 1903 Hansen,* who discovered the organism, declared that no one had yet culti- vated it. Bordoni-Uffreduzzif was able to cultivate a bacillus which par- took of the staining peculiarities of the lepra bacillus as it appears in the tissues, but differed in morphology. CzaplewskiJ confirmed the work of Bordoni-Uffredozzi, and. Fig. 283.—Section of one of the nodules from the patient sho
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Cultivation 697 this bacillus upon artificially prepared media, but in 1903 Hansen,* who discovered the organism, declared that no one had yet culti- vated it. Bordoni-Uffreduzzif was able to cultivate a bacillus which par- took of the staining peculiarities of the lepra bacillus as it appears in the tissues, but differed in morphology. CzaplewskiJ confirmed the work of Bordoni-Uffredozzi, and. Fig. 283.—Section of one of the nodules from the patient shown in Fig. 285, stained by the Weigert-Gram method to show the lepra bacilli scattered through the tissue and inclosed in the large vacuolated "; Magnified 1000 diameters. described a bacillus supposed to be the lepra bacillus, which he succeeded in cultivating from the nasal secretions of a leper. The bacillus was isolated upon a culture-medium consisting of glycerinized serum without the addition of salt, peptone, or sugar. The mixture was poured into Petri dishes, coagulated by heat, and sterilized by the intermittent method. * KoUe and Wassermann's "Handbuch der pathogenen Mikroorganismen," n, p. 184, 1903. t "Zeitschrift f. Hygiene," etc., 1884, in. t "Centralbl. f. Bakt. und Parasiteuk.," Jan. 31, 1898, vol. xxin, Nos. 3 and 4, p. 97-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original McFarland, Joseph, 1868-. Philadelphia and London, W. B. Saunders Company
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