. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. 114 V. p. GILEV The plate of gelatin with the objects included in it is separated from the oil-cloth and cut for rectangu- lar blocks. The blocks are glued up with 40 "o solu- tion of gelatin to the old methacrylic blocks sharp- ened in the form of a truncated pyramid and placed for several hours into a thermostat (37'C) for com- plete drying. Immediately before cutting in a microtome the blocks are sharpened in such a way that the square of the section will not exceed mm-. The blocks
. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. 114 V. p. GILEV The plate of gelatin with the objects included in it is separated from the oil-cloth and cut for rectangu- lar blocks. The blocks are glued up with 40 "o solu- tion of gelatin to the old methacrylic blocks sharp- ened in the form of a truncated pyramid and placed for several hours into a thermostat (37'C) for com- plete drying. Immediately before cutting in a microtome the blocks are sharpened in such a way that the square of the section will not exceed mm-. The blocks are kept in cans with tight caps. Cutting is effected with a glass knife (without fluid). In our work we used a microtome of Danon and Kcllenberger. The long side of the block should be parallel to the sharp edge of the knife. The blocks just dried are cut worse when the weather is dry; in this case it is better to cut them not earlier than in 24 hours after they were exposed to the air. In the case of considerable humidity of the air and blocks, it is recommended to dry them some time before cutting. Position of the knife in cutting gelatin blocks is usually, to some extent, steeper than in cutting methacrylic blocks. The rate of cutting is approximately I section per I sec. It is not difficult to obtain sections /< thick. Usually the sections are formed in the shape of endurable un- disintegrating ribbons. These ribbons are sorted out and individual sections or groups of sections are trans- ferred with the aid of a thin filament into drops of water (or better of 2 % solution of acetic acid) lying on a Parlodion film that floats on the surface of water (temperature of about 37-40 C) filling a Koch cup. But better results are obtained when the sec- tions are placed into water of room temperature, which is subsequently heated up to 37-40 C. Expanding, the sections in most cases lose their bonds with each other and, therefore, not always a series of sections ma
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