. The Biological bulletin. Biology; Zoology; Marine biology. DYNAMICS OF ECDYSONE 327 total ecdysone in the entire blood could scarcely exceed about ixg, , 15% of a Sarcophaga unit for standard test abdomens. 4. Extraction of blood ecdysone In order to bring this low titer within the range of the biological assay, we undertook the extraction and partial purification of blood ecdysone as described under Methods. The extract was redissolved in distilled water as a tenfold con- centrate of the original blood, and 10 fA were injected into each of a series of standard test abdomens. X LJ
. The Biological bulletin. Biology; Zoology; Marine biology. DYNAMICS OF ECDYSONE 327 total ecdysone in the entire blood could scarcely exceed about ixg, , 15% of a Sarcophaga unit for standard test abdomens. 4. Extraction of blood ecdysone In order to bring this low titer within the range of the biological assay, we undertook the extraction and partial purification of blood ecdysone as described under Methods. The extract was redissolved in distilled water as a tenfold con- centrate of the original blood, and 10 fA were injected into each of a series of standard test abdomens. X LJ Q 3 < ID. ECDYSONE INJECTED (yug) Figure 2A. The developmental responses (computed in terms of the puparium index) are plotted as a function of the amount of injected a-ecdysone. The curves correspond to abdomens ligatured after the indicated hours of exposure to dry conditions. For description of "standard test abdomens," see text. Figure 2B. The inset in the upper left corner records the amount of injected ecdysone required for a 50% response of each type of Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-; Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report; HighWire Press. Lancaster, Pa. [etc. ] : Lancaster Press, inc. [etc. ]
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