. Bacteria in relation to plant diseases. Bacteria; Plant diseases. 184 BACTERIA IN RELATION TO PLANT DISEASES. Result.—Very successful (Bull. 12, pi. 1, figs. 6, 7, and pi. 2, fig. 2). First signs appeared on third or fourth day; weather hot, 280 to 320 C. The organism swarming in tissues of affected plants was a short, motile rod. June 23.—Three old potato tubers and 3 cucumber fruits were used for these inoculations (4 inoculations, 2 checks). The cucumbers were 7 inches long by 2 inches thick, green and firm. The potato tubers were large, smooth and white, probably Burbank, kept under bell
. Bacteria in relation to plant diseases. Bacteria; Plant diseases. 184 BACTERIA IN RELATION TO PLANT DISEASES. Result.—Very successful (Bull. 12, pi. 1, figs. 6, 7, and pi. 2, fig. 2). First signs appeared on third or fourth day; weather hot, 280 to 320 C. The organism swarming in tissues of affected plants was a short, motile rod. June 23.—Three old potato tubers and 3 cucumber fruits were used for these inoculations (4 inoculations, 2 checks). The cucumbers were 7 inches long by 2 inches thick, green and firm. The potato tubers were large, smooth and white, probably Burbank, kept under bell-jars. Same culture used as for inoculations of June 15. Result.—The cucumber fruits rotted, but no plates were poured. In the worst injured potato the bacteria, which were numerous along the line of the punctures, extended scarcely 1 mm. beyond; starch intact. July 11.—Two cucumber fruits under a bell-jar were inoculated from tube No. 4, July 8. Result.—One of the fruits rotted, but no plates were poured. The bacteria filling the interior were short, actively motile rods (see footnote on p. 174). July II.—The following plants were inoculated: Potato (7 plants), tobacco (Connecticut seed leaf, 2 plants), cucumber (4 plants), petunia (2 plants, 6 shoots), Solatium nigrum (1 plant). Datura stramonium (2 plants). Datura tatula (?) (1 plant), Physalis crassifolia (1 plant, several shoots). Twenty-five inoculations were made by needle-pricks on a very hot, sunny day. The inoculated parts were shaded. The cultures used were tubes of beef-broth Nos. 1, 2, 3, 4, 5, 6, and 8 of July 8, from sugared peptone-water in fer- mentation-tubes (descended from egg-plant from Charleston, S. C). The potatoes were tall shoots, half-grown in 8-inch pots, and each plant was inoculated from a separate tube (about 30 pricks in all). Numerous check-plants were held. The tobacco was inoculated (100 pricks) in a big leaf. The Datura tatula (?) was a young, vigorous plant. The plants of D. stra
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