. A compilation of culture media for the cultivation of microorganisms. Bacteriology -- Cultures and culture media; Microorganisms. 710 CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS Variants: Harvey prepared a similar me- dium as follows: (1) Dissolve g. agar in cc. of water. (2) Boil cc. water and g. garden soil for 2 hours. (3) Pour off the turbid fluid. (4) Mix talc with (3). (5) Filter until clear thru thick filter paper. (6) Mix cc. of (5) and cc. of (1). (7) The reaction should be acid to phenolphthalein. (8) Add g. glucose to (7). (9) Tube
. A compilation of culture media for the cultivation of microorganisms. Bacteriology -- Cultures and culture media; Microorganisms. 710 CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS Variants: Harvey prepared a similar me- dium as follows: (1) Dissolve g. agar in cc. of water. (2) Boil cc. water and g. garden soil for 2 hours. (3) Pour off the turbid fluid. (4) Mix talc with (3). (5) Filter until clear thru thick filter paper. (6) Mix cc. of (5) and cc. of (1). (7) The reaction should be acid to phenolphthalein. (8) Add g. glucose to (7). (9) Tube. (10) Sterilization not specified. References: Tanner (1919 p. 49), Harvey (1921-22 p. 105). 2197. Lohnis' Mannitol Soil Infusion Agar Constituents: 1. Water cc. 2. Soil g. 3. Mannitol () g. 4. K2HPO4 () g. 5. Agar () g. Preparation: (1) Heat g. of good rich garden soil with a liter of tap water for 30 minutes in the autoclave under pres- sure of 1 atmosphere or boil with 2 liters of water over a free flame. (2) Pour off the turbid liquid. (3) Mix talc with the liquid. (4) Filter thru a double filter paper. (5) Make up the volume to cc. if necessary. (6) Dissolve 3, 4 and 5 in (5). (7) Tube. Sterilization: Sterilize in the autoclave or stQamer. Use: Isolation of nodule bacteria. Reference: Lohnis (1913 p. 112). 2198. Perotti's Dicyandiamide Soil Infusion Agar Same as medium 1392 but solidified by the addition of agar. ^. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Levine, Max, 1889-1967; Schoenlein, Henry W. Baltimore, The Williams & Wilkins Company
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