. Pathological technique; a practical manual for workers in pathological histology and bacteriology. ge numbers. White mice die in twenty-four hours to three days. Rab-bits inoculated in the ear vein and guinea-pigs inoculated inthe peritoneal cavity may die within twenty-four hours. Subcutaneous inoculation of the animals last named leadsonly to local suppuration. The lesions produced by thisorganism consist in marked congestion of the superficialveins, hemorrhage into the lymphatic glands, and enlarge-ment and softening of the spleen. In the guinea-pig ahemorrhagic condition of the suprarena
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. ge numbers. White mice die in twenty-four hours to three days. Rab-bits inoculated in the ear vein and guinea-pigs inoculated inthe peritoneal cavity may die within twenty-four hours. Subcutaneous inoculation of the animals last named leadsonly to local suppuration. The lesions produced by thisorganism consist in marked congestion of the superficialveins, hemorrhage into the lymphatic glands, and enlarge-ment and softening of the spleen. In the guinea-pig ahemorrhagic condition of the suprarenal capsules is present. PATHOGENIC BACTERIA AND FUNGI. 333 and in the peritoneal cavity there may be a small amountof clear, rather viscid fluid containing the bacilli in largenumbers. The organs on microscopic examination may show pecu-liar areas in which the cells and nuclei are shrunken and inwhich the bacilli are aggregated. Occurrence.—This organism or closely related forms maybe met with in broncho- or lobular pneumonia and in inflam-matory conditions of the air-passages generally. It may. Fig. 90.—Bacillus mucosus- capsulatus, from a culture; x 1000 (Wright and Brown). also be present in the upper air-passages of healthy individ-uals. It has been observed in inflammations of the middleear, in empyema, meningitis, endocarditis, peritonitis, and inpus formations. In fatal infections the blood-stream may befound invaded by the organism. It is held by some bacteri-ologists that the members of this group may be the infectiveagents in genuine croupous pneumonia in rare of this group have been found in the soil,in the air, and in contaminated water. The followingmethod is recommended for staining the capsules in sections: 334 PATHOLOGICAL TECHNIQUE. i. Stain for twenty-four hours in the incubator in the fol-lowing solution: Concentrated alcoholic solution of methyl-violet, 50;Distilled water, 100; Glacial acetic acid, 10. 2. Wash out in a 1 per cent
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