Archive image from page 132 of Cytological technique; the principles underlying. Cytological technique; the principles underlying routine methods cytologicaltechn00bake Year: 1960 THE ACTION OF MORDANTS 113 4' ' ♦ O Q ♦ 0 'Q ' //. 5. Structural formulae for hydrated aluminium ions, drawn in perspective. The ion on the left carries three positive charges. That on the right has lost a hydrogen ion and carries two positive charges. nuclei (aluminium, oxygen, and hydrogen, together), may be re- garded as holding 3 positive charges at the moment of its forma- tion. The 6 water molecules
Archive image from page 132 of Cytological technique; the principles underlying. Cytological technique; the principles underlying routine methods cytologicaltechn00bake Year: 1960 THE ACTION OF MORDANTS 113 4' ' ♦ O Q ♦ 0 'Q ' //. 5. Structural formulae for hydrated aluminium ions, drawn in perspective. The ion on the left carries three positive charges. That on the right has lost a hydrogen ion and carries two positive charges. nuclei (aluminium, oxygen, and hydrogen, together), may be re- garded as holding 3 positive charges at the moment of its forma- tion. The 6 water molecules, however, do not all remain intact. One of them, at least, tends to lose a hydrogen ion, which joins a molecule of the solvent water to form a hydronium ion, HgO, and thus departs. Since it carries off a positive charge, the com- plex ion (fig. 6, right) is only doubly charged; but the solution, having gained a hydronium ion, has become acid. Thus the pH of an M/40 solution of aluminium sulphate in 60 ethanol is 3-08. When purpurine is dissolved in water or in solutions of ethanol in water, it behaves like a phenol; that is to say, like a weak acid. A saturated solution in 60 ethanol has a pH of 5-20. Some of the hydrogens of the -OH groups are ionized and lost, and the dye is now negatively charged. In the structural formula shown here, O 1- OH OH O O Ionized purpurine
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