Ecological investigations of the tundra Ecological investigations of the tundra biome in the Prudhoe Bay region, Alaska . ecologicalinvest00brow Year: 1975 154 At the end of a collection period, the appa- ratus was taken from the animal, and saliva was forced from the forage sample by squeezing. Wet weights of forage and saliva samples were deter- mined, and the forage was subdivided for deter- minations of plant species composition, dry matter content, and chemical composition. Identification of plant material in rumen and esophageal egesta samples Rumen and esophageal egesta samples were pr
Ecological investigations of the tundra Ecological investigations of the tundra biome in the Prudhoe Bay region, Alaska . ecologicalinvest00brow Year: 1975 154 At the end of a collection period, the appa- ratus was taken from the animal, and saliva was forced from the forage sample by squeezing. Wet weights of forage and saliva samples were deter- mined, and the forage was subdivided for deter- minations of plant species composition, dry matter content, and chemical composition. Identification of plant material in rumen and esophageal egesta samples Rumen and esophageal egesta samples were preserved in 80%%%% ethanol. Since chlorophyll is removed from plant parts by alcohol, identifica- tions were made within 2 days of sampling to determine live and dead plant parts. Samples in the preservative were added to an enamel tray (25x40 cm), and 200 point identifications were made with a binocular (xlO) as described by Gait et al. (1969); Gaare et al. (1970); Gaare and Skogland (1971). For comparison with samples of rumen con- tents and esophageal egesta, the plant species composition of communities on which the ani- mals were grazing was also determined (see Description of vegetation, above). Estimation of plant biomass and primary production Biomass estimates were made by clipping all vegetation above the moss layer in 30x30 cm plots. Clipped vegetation was sorted into green (live) and dead material, weighed, and dried at 50° C. Above-ground vascular production was esti- mated through the season from changes in biomass; total yearly above-ground production was estimated, assuming that peak green biomass represents total annual production (Tieszen 1972). Chemical analyses Dry matter content of vegetation, rumen samples, and esophageal egesta samples were determined by drying to constant weight in a forced air oven at 40-55° C. Estimates of cell contents, hemicelluiose, and iigno-cellulose in vegetation were made using the acid detergent/neutral detergent tech- nique of
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