. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SETTLEMENT-CUE PERCEPTION IN VELIGERS 71. Figure 2. Veliger larvae of Phi:\lilln vhtigM- stained with DASPEI. (top) Bright-field image, (middle) Same specimen photographed with fluo- rescent light, (bottom) Enlargement of anterior region of the same speci- men showing DASPEI staining in 5-6 cells of the apical sensory organ. Scale bars, top and middle = 50 /im. bottom = 25 yum. irradiated. We conclude that exposure of the stained ASO to intense excitatory irradiation for 20 min resulted in photo- ablation of the ASO cells, a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SETTLEMENT-CUE PERCEPTION IN VELIGERS 71. Figure 2. Veliger larvae of Phi:\lilln vhtigM- stained with DASPEI. (top) Bright-field image, (middle) Same specimen photographed with fluo- rescent light, (bottom) Enlargement of anterior region of the same speci- men showing DASPEI staining in 5-6 cells of the apical sensory organ. Scale bars, top and middle = 50 /im. bottom = 25 yum. irradiated. We conclude that exposure of the stained ASO to intense excitatory irradiation for 20 min resulted in photo- ablation of the ASO cells, and that subsequent loss of the ability of these larvae to respond to the metamorphic in- ducer contained in coral seawater is evidence that the irra- diated cells were the site of receptors for the inducer. These larvae were otherwise unharmed. They continued to swim normally in FSW. and they had not lost the capacity to metamorphose in response to artificial inducers (see below). The yellow DASPEI fluorescence disappeared from the ASO following excitatory illumination, although it persisted in other regions of the larval body. When larvae whose ASO cells had been stained with DASPEI and exposed for 20 min to fluorescent irradiation and thus photobleached were placed in a seawater solution of acridine orange, the ASO cells uniquely absorbed the dye and emitted a green fluorescence (Fig. 5). Comparable stain- ing in other cells and organisms has been found specific to induced cell death (Delic et a!.. 1991; Abrams et al., 1993). ASO cells of untreated larvae did not take up acridine orange. These observations support the conclusion that DASPEI staining followed by fluorescent irradiation of the ASO cells led to their ablation. When larvae that had undergone the photoablation treat- ment described above were exposed to seawater containing 20 mM cesium ion for 20 min and then transferred to FSW. they underwent normal metamorphosis in large numbers within 24 h (Fig. 6). Similarly t
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