. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. '10 20 30 Time after activation (min) Figure 6. Migration of"female pronuclei in normal (a) and binudeate (b) eggs on activation at 26°C, as indicated by decrease in the distance (ordinates) between pronuclei and the centers of the eggs. Stars in (b) mark fused nuclei. Data in Figures 4 and 6 are derived from a single batch. Note that the migration starts later and is slower than that observed in fertilized eggs (cf. Fig. 4).. Figure 7. A pair of partial nuclei in activated eggs treated with 1 5 min after
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. '10 20 30 Time after activation (min) Figure 6. Migration of"female pronuclei in normal (a) and binudeate (b) eggs on activation at 26°C, as indicated by decrease in the distance (ordinates) between pronuclei and the centers of the eggs. Stars in (b) mark fused nuclei. Data in Figures 4 and 6 are derived from a single batch. Note that the migration starts later and is slower than that observed in fertilized eggs (cf. Fig. 4).. Figure 7. A pair of partial nuclei in activated eggs treated with 1 5 min after activation. Numerals indicate the time after activation. Both nuclei remained separated, but their nuclear envelopes still broke down (40 mm) and reformed (79 min). 26°C. Scale bar = 25 tiple nuclei. Thanks to the natural transparency of Cly- pcustcr eggs, we can easily detect, with a low power mi- croscope, batches of eggs including those with partial nu- clei. Using eggs with partial nuclei may give us some insights into the process of nuclear migration and nuclear fusion in echinoderm eggs. Literature Cited Boveri, I. 1918. Zwei Fehlerquellen bei merogonischen und die Ent- wicklungsfahigkeit merogonischer und partiell-merogonischer Seei- gelbastarde. Arch. Entwicklungsmech. 44: 419-471. Chambers, R., and E. L. Chambers. 1961. Explorations into the Nan/re / the Living Cell Harvard University Press, Cambridge. Hamaguchi, M., and Y. Hiramoto. 1986. Analysis of the role of astral rays in pronuclear migration in sand dollar eggs by the Colcemid- UV methods. Dt-\: Growth Differ. 28: 143-156. Mar, II. 1980. Radial cortical fibers and pronuclear migration in fer- tilized and artificially activated eggs of Lytecliinus pictus. Dev. Biol. 78: 1-13. Schatten, G., and 1981. Effects of motility inhibitors during sea urchin fertilization. E.\p. Cell Res. 135: 311-330. Sluder, G. 1979. Role of spindle microtubules in the control of cell c\cle timing. ./ Cell Biol 80: 674-691
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