. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ARBACIA SPERM AGING 255 and allowed to stand in 100- or 250-ml. beakers at room temperatures. Periodically the suspensions were thoroughly stirred and portions tested by centrifugation for motility, and in serial dilution for fertilizing capacity. Sperm concentration was determined by absorptiometry at each interval and confirmed occasionally by direct counts. Under the conditions of these experiments (25 in all) sperm concentration as well as motility and fertilizing capacity declined with time (Fig. 1). In different 100 90
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ARBACIA SPERM AGING 255 and allowed to stand in 100- or 250-ml. beakers at room temperatures. Periodically the suspensions were thoroughly stirred and portions tested by centrifugation for motility, and in serial dilution for fertilizing capacity. Sperm concentration was determined by absorptiometry at each interval and confirmed occasionally by direct counts. Under the conditions of these experiments (25 in all) sperm concentration as well as motility and fertilizing capacity declined with time (Fig. 1). In different 100 90 so 7O 60 o so 4O 30 20 10. 8-O ,HCL 7-5 7O 65 6 O PH FIGURE 2. Effects of acid and carbon dioxide on Arbacia sperm motility. Sperm were shed into 250 ml. of sea water to 18 X 10" sperm/ml., then divided into three equal portions. One portion served as control, the second was acidified by bubbling with CO3 in air, and the third was acidified with HC1. The motility of the three portions was determined simul- taneously by centrifugation. The process was repeated at different pH values with fresh sperm shed from the same male. The data are presented as percentage of the control sperm motility. samples the average rate of decrease in concentration varied from X 106 sperm/ml, lost each hour to 5 X 106 sperm/ml, lost each hour. There was no evidence that the loss of sperm resulted from sedimentation or the adherence of sperm to the walls of the container. The decrease in sperm concentration had to be considered in motility and fertilization capacity determinations, so concentration was determined and new portions killed for motility determination at each time Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Wo
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
