. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTEIN METABOLISM IN ABALONE LARVAE 179 co O <J <D Q. O co a. 5- I3' 3. 12 16. > lOOOi .c w II w c c ^ O 'm 600 O) Q. 400 4 8 12 Time (minutes) 16 Figure 2. Determination of the absolute rate of protein synthesis in 7-day-oId veliger larvae of fialiotis rufescens. (A) Specific activity of intracellular glycine in the free amino acid pool following transport of '4C-glycine. Each point represents the specific activity determined by high-performance liquid chromatography using fluorescence detection (moles of gly
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTEIN METABOLISM IN ABALONE LARVAE 179 co O <J <D Q. O co a. 5- I3' 3. 12 16. > lOOOi .c w II w c c ^ O 'm 600 O) Q. 400 4 8 12 Time (minutes) 16 Figure 2. Determination of the absolute rate of protein synthesis in 7-day-oId veliger larvae of fialiotis rufescens. (A) Specific activity of intracellular glycine in the free amino acid pool following transport of '4C-glycine. Each point represents the specific activity determined by high-performance liquid chromatography using fluorescence detection (moles of glycine) and measuremenl by liquid scintillation counting of radioactivity in the glycine peak collected from the chromatographic eluent. (B) Radioactivity in the irichloroacetic acid (TCA)-insoluble frac- tion (protein). (C) Rate of protein synthesis after correcting the TCA- msoluble traction for the change in intracellular specific activity of L4C- glycine. the mole percent of glycine in protein, and the average mole- percent corrected molecular weight of amino acids in larval protein (Table I). glycine peak. All measurements of radioactivity (as counts per min. CPM) were corrected for quenching and converted to total disintegrations per min (DPM). By correcting for the change in the specific activity of glycine in the free amino acid pool (Fig. 2A), the incorporation rate of '4C-glycine (Fig. 2B) could be converted to the total amount of glycine incorporated into protein (both I2C- and 14C-glycine). The value for the incorporation of total glycine into protein was then converted to an absolute rate of protein synthesis (Fig. 2C) by determining the mole-percent of glycine in larval protein and the mole-percent-corrected molecular weight of all amino acids in larval protein of H. rufescens (Table I). The components required for the calculation of the absolute rate of protein synthesis are MW _ where Jt5 is the rate of protein synthesis, MW/t is the mole- percent-corrected molecu
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
