. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. DISEASES CAUSED BY SPIROCHiBTES 607 original blood for as long as forty days, and call attention to the fact that the length of time for which they may be kept alive depends to a great extent upon the stage of fever at which the blood is removed from the patient. They do not, however, beUeve that extensive multiplica- tion, or, in other words, actual cultivation, had taken place in their experiments. Norris, Pappenheimer, and Flournoy, on the other hand, have obtained positive evidence
. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. DISEASES CAUSED BY SPIROCHiBTES 607 original blood for as long as forty days, and call attention to the fact that the length of time for which they may be kept alive depends to a great extent upon the stage of fever at which the blood is removed from the patient. They do not, however, beUeve that extensive multiplica- tion, or, in other words, actual cultivation, had taken place in their experiments. Norris, Pappenheimer, and Flournoy, on the other hand, have obtained positive evidence of multiphcation of the spirochsetes in fluid media. They obtained their cultures by inoculating a few drops of spirochaetal rat blood into 3 to 5 of citrated human or rat blood. Smears made froin these tubes, after preservation for twenty-four hours at room temperatm-e, showed the microorganisms in greater number. Fig. 134.—Spirochete of Relapsing Fever. (From preparation furnished by Dr. G. N. Calkins.) than in the original infected blood. A similar multiphcation could be observed in transfers made from these "first-generation" tubes to other tubes of citrated blood. Attempts at cultivation for a third generation, however, failed. Noguchii has lately successfully cultivated the spirochaete of Ober- meier in ascitic fluid containing a piece of sterile rabbit's kidney and a few drops of citrated blood under anaerobic conditions. Four different, probably distinct varieties of spirochaete have been described in cormection with relapsing fever, all of which have been cultivated by Noguchi by means of this method. The first is known as the spirochaete of Obermeier mentioned above. Probably distinct ' Noguchi, Jour. Exp. Med., xvii, Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Hiss, Ph
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