. Bacteria in relation to plant diseases. Bacteria; Plant diseases. WILT-DISEASES OF TOBACCO. 243 Nicotiana rustica. is not attacked, and the following varieties of N. tabacum are not much subject: Ohasama, Taketadate, Mitsuke, Kentucky White, Green River Prior. Inoculations succeeded on Physalis minimum, Capsicum Ion gum, Amaranthus gangeiicus, and Polygonum tinctorum; they failed on Solatium mclongena, Lycopersicum esculentum, and Physalis alkekengi. No details are given as to methods of inoculation, phenomena produced, or number of experiments. Nitrogenous fertilizers predispose to disease;
. Bacteria in relation to plant diseases. Bacteria; Plant diseases. WILT-DISEASES OF TOBACCO. 243 Nicotiana rustica. is not attacked, and the following varieties of N. tabacum are not much subject: Ohasama, Taketadate, Mitsuke, Kentucky White, Green River Prior. Inoculations succeeded on Physalis minimum, Capsicum Ion gum, Amaranthus gangeiicus, and Polygonum tinctorum; they failed on Solatium mclongena, Lycopersicum esculentum, and Physalis alkekengi. No details are given as to methods of inoculation, phenomena produced, or number of experiments. Nitrogenous fertilizers predispose to disease; pot- ash salts do not. Early planting is the best protec- tion. Through the courtesy of Dr. Uyeda, who sent alcoholic material, I was enabled to study his dis- ease in serial sections. The bacteria closely resemble Bad. solanacea- rum in general appearance. They are enormously abun- dant in vessels of the wood, in inner and outer phloem, in the cortical parenchyma, and in the pith. I also found them to have made cavities resembling those due to Bad. solanacearum. They stained deep red with carbol fuchsin and were quite uniform in appearance. Spores were not observed. (Compare figs. 131 and 132 with those made from stems of the North Carolina to- bacco, figs. 116 and 117.) Also, with bacteria taken from this Japanese tobacco stem I made several attempts to stain the flagella. At first I got no indications of peritrichiate rods and many faint indications of Fig Fig. I33.{ polar flagella; nevertheless no well-stained ones, such as could be relied on for Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Smith, Erwin F. (Erwin Frink), 1854-1927. Washington, Carnegie Inst.
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