. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 50 T. BJORKMAN. to- Figure 1. Detail of top and bottom of chamber showing how root tip was positioned and accessible to vibrating probe while preventing loss of solution when turned. Full description in Materials and Meth- ods. Vibrating probe The chamber to hold the seedlings during the experi- ment had to meet several requirements: (1) the surface at the root tip had to be clear to allow precise microscopic observation of the root tip and the vibrating probe; (2) there had to be a means of positioning the seedling within t
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 50 T. BJORKMAN. to- Figure 1. Detail of top and bottom of chamber showing how root tip was positioned and accessible to vibrating probe while preventing loss of solution when turned. Full description in Materials and Meth- ods. Vibrating probe The chamber to hold the seedlings during the experi- ment had to meet several requirements: (1) the surface at the root tip had to be clear to allow precise microscopic observation of the root tip and the vibrating probe; (2) there had to be a means of positioning the seedling within the chamber during the experiment; (3) there had to be access for the vibrating probe and for a calibrating elec- trode; and (4) the chamber needed to be rotated 90° in either direction from the vertical without draining the solution from it. The seedlings were held in a plexiglass chamber 10 x 10 mm at the base and 15X15 mm at the top; height 50 mm (Fig. 1). The upper surface had an opening large enough to insert the seedling; this opening was then cov- ered with a lid using lanolin to make a strong and water- tight seal. The lid had a 1 -mm orifice through which a 24 gauge stainless steel cannula was passed and inserted into the endosperm of the seedling. The cannula needed to be ;o that it displaced a minimum amount of solution L> position the root tip. The orifice was filled ;e a flexible and watertight seal. The exler ,: la was attached to a micro- manipulator, precise positioning of the seedling withi Three mm fro f the chamber, a diameter hole < side to allow access for the vibrating pn uali ng electrode. This configuration allovv< of the apical 3 mm of the root, the region . ; his study. The holes were large enough that a probe could successfully be passed through without touching the sides; probe vibra- tion was axial. When the top of the chamber was sealed, solution would not drain out these holes, even when the chamber was turned on its side. While th
Size: 1754px × 1424px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
