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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AMEBOCYTE AGGREGATION IN LIMULUS 555. 4b. 90 105 120 TI M E ( sec ) FIGURE 4 (continued). exemplify the typical morphological alterations of aggregation (Dumont, Ander- son and \Yinner, 1966). Not infrequently, EDTA preparations have amebocytes that are more round than elliptical and a few free intact granules are present out- side of the cells. Although EDTA at 17 ± 6 HIM markedly inhibits amebocytes aggregation, this inhibition is perhaps more aptly termed a retardation of aggregation because, with time, some aggregation t
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AMEBOCYTE AGGREGATION IN LIMULUS 555. 4b. 90 105 120 TI M E ( sec ) FIGURE 4 (continued). exemplify the typical morphological alterations of aggregation (Dumont, Ander- son and \Yinner, 1966). Not infrequently, EDTA preparations have amebocytes that are more round than elliptical and a few free intact granules are present out- side of the cells. Although EDTA at 17 ± 6 HIM markedly inhibits amebocytes aggregation, this inhibition is perhaps more aptly termed a retardation of aggregation because, with time, some aggregation takes place in EDTA. During the first 2 min after addi- tion of amebocytes in EDTA to the cuvette, a slight rise (0—2%) in transmission frequently occurs, but over 5 min period increases in transmission are usually significant (0-15%). Furthermore, after stirring on the aggregometer for i min, microscopic examination of amebocytes in EDTA reveals aggregates even when no rise in transmission is observed. These aggregates range from small (approxi- mately 2-20 cells) to medium sized (100 cells), and the amebocytes within these aggregates retain their identity with pseudopods evident only on those cells at the periphery of the aggregates. Lower concentrations on EDTA (in the range. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology