. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GUT CL PV LBWM INT. FIGURE 1. Preparation of isolated pharyngeal retractor muscles (PRM) from everted Thyonc; (a) The relationship between the PRM and the longitudinal body wall muscle (LBWM). Note the calcareous lantern (CL), and the relationship between the tentacular canals (TC) and the anterior insertion of the PRM. Other abbreviations are PV, polian vesicle; WR, water ring; INT, introvert, (b) The anterior end of the PRM is freed by inserting a scissors in the tentacular canals and cutting a pie-shaped piece from the os


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GUT CL PV LBWM INT. FIGURE 1. Preparation of isolated pharyngeal retractor muscles (PRM) from everted Thyonc; (a) The relationship between the PRM and the longitudinal body wall muscle (LBWM). Note the calcareous lantern (CL), and the relationship between the tentacular canals (TC) and the anterior insertion of the PRM. Other abbreviations are PV, polian vesicle; WR, water ring; INT, introvert, (b) The anterior end of the PRM is freed by inserting a scissors in the tentacular canals and cutting a pie-shaped piece from the ossicle. Threads are tied near each attachment of the PRM : one on the ossicle and one on the LBWM ; (c) The isolated PRM, tied to a stainless steel hook, and immersed in the testing bath (not shown). The upper thread is attached to a force-displacement transducer. After the bulk of the viscera have been removed, the lantern, retractor muscles, and the body wall are washed with sea water. After sufficient washing, the animal will recover without undergoing either autotomy of the pharyngeal retractor muscles (PRMs) or softening of the introvert wall. If the isolated PRMs are to be prepared, the freshly everted animal is washed with anesthetic solution instead of sea water. The lantern attachment of the PRM can then be freed by inserting the sharp point of a fine scissors in the exposed tentacular canal on either side of the anterior insertion of the muscle and cutting a wedge shaped piece from the calcareous plate of the lantern. Once the piece of lantern has been cut away from the introvert, the anterior end of the PRM is detached (Fig. Ib). A thread is attached to the lantern Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laborat


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology