. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BLOOD OF PODOCLAVELLA MOLUCCENSIS 673 Sigillina cyanca blood cells lysed in ethanol gave a bright blue lysate with the spec- trum shown in Figure 2. After exhaustive extraction with ethanol, no material was extracted into acidified ethanol. The ethanol extract gave a blue solid on evaporation to dryness. Upon partitioning between hexane and 85% methanol, 75% of this solid went into the hexane and the remainder into the methanol. The spectra of these two solutions are given in Figure 2. The blue extract had no detectable CD.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BLOOD OF PODOCLAVELLA MOLUCCENSIS 673 Sigillina cyanca blood cells lysed in ethanol gave a bright blue lysate with the spec- trum shown in Figure 2. After exhaustive extraction with ethanol, no material was extracted into acidified ethanol. The ethanol extract gave a blue solid on evaporation to dryness. Upon partitioning between hexane and 85% methanol, 75% of this solid went into the hexane and the remainder into the methanol. The spectra of these two solutions are given in Figure 2. The blue extract had no detectable CD. Cells of the orange form of Polycarpa pedunculata were also lysed in ethanol and exhaustively extracted with that solvent, giving a bright yellow, solution. Sub- sequent extraction with acidified ethanol also gave a yellow solution. Figure 2 shows the absorption and CD spectra of both fractions. Not enough blood was isolated from specimens of Polycarpa pednnculata with white tests for these types of studies. Blood plasma Absorption and CD spectra of the plasma of Podoclavella tnolucccnsis and the two forms of Polycarpa pednnculata are shown in Figure 2. Unfortunately the Sigillina cyanca plasma was destroyed before its spectrum was measured. The elution pattern of Podoclavella ntoluccensis plasma chromatographed through Sephadex G75 is given in Figure 4. A colorless protein band that gave a positive biuret test but a negative PAS test for saccharide was eluted in the first 10 ml frac- tion following the exclusion volume of the column. Fractions 2 and 3, part of the same band as fraction 1, were yellow and tested positive for protein and negative for saccharide. Fractions 9—12 came under the second major elution band. They were yellow and tested negative for protein but positive for saccharide. The absorption spectra for the individual fractions under this chromatographic band varied across the band (Fig. 5). The CD spectrum for fraction 10 is included in Figure 5. 4,9-3-78. 1
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
