Injury, recovery, and death, in relation to conductivity and permeability . a water, which iscontained in the apparatus, and in the cell walls betweenthe protoplasmic masses. The results of the experi-ment are shown in Fig. 80. In order to see how the evaporation of the ether ^ Since ether, chloroform, and alcohol deteriorate on standing, espe-cially when in contact with metal or with cork stoppers, special caremust be taken to obtain pure reagents. Those used were Kahlbaumsor Squibbs. C/. Baskerville (1913). ^C/. Osterhout (1913, A; 1916, A). »C/. Osterhout (1919, D). *A11 readings were made


Injury, recovery, and death, in relation to conductivity and permeability . a water, which iscontained in the apparatus, and in the cell walls betweenthe protoplasmic masses. The results of the experi-ment are shown in Fig. 80. In order to see how the evaporation of the ether ^ Since ether, chloroform, and alcohol deteriorate on standing, espe-cially when in contact with metal or with cork stoppers, special caremust be taken to obtain pure reagents. Those used were Kahlbaumsor Squibbs. C/. Baskerville (1913). ^C/. Osterhout (1913, A; 1916, A). »C/. Osterhout (1919, D). *A11 readings were made at 18° C. or corrected to this temperature. 184 ANESTHESIA 185 from the solution influenced the result, another experi-ment was performed in which the solution was renewedevery 5 minutes during the first 60 minutes, and there-after every 15 minutes. In this way the concentration ofether was kept more nearly constant. It was then foundthat the resistance rose as before, but did not fall during A Sea Water. B Ether 1% (= .099M) solution not 1% solution 100- MINUTES 500 Fia. 80.—Curve A shows the net electrical resistance of Laminaria agardhii in sea water; Bin sea water containing 1% ether by volume (.099 M) from which the ether was allowed toevaporate in an open dish; C in the same mixture in which the concentration of ether was maintained by frequent renewal. the first 80 minutes, and after this fell very slowly, sothat after 300 minutes it was still 80 ohms above that ofthe control. At this point, the experiment was discon-tinued. The results are shown in Fig. 80. In order to see whether the effect of the anestheticcould be quickly reversed, some tissue was kept in seawater containing M ether for 50 minutes (thesolution being renewed every 5 minutes). During thistime the resistance rose to It was then placedin sea water. At the end of 10 minutes the resistancehad fallen to 100%. It was then left in sea water contain-ing M ether (the


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Keywords: ., bookcentury1900, bookdecade1920, booksubjectphysiology, bookyear1