. The Biological bulletin. Biology; Zoology; Marine biology. COMPARATIVE P'- UPTAKE BY H. ATRA 373 mals were removed from their containers after 6, 9, 12, 25, 36, and 48 hours. The organs of the viscera were removed and separately analyzed for their P'- content. The integument was also subjected to phosphorus analysis. Coelomic fluid was not withdrawn from these sterilized animals. Dehydrating, ashing and counting techniques used for the tissue components of this portion of the study were carried out as ASH WEIGHT INTEGUMENT (grams) Figure 4. The uptake of P'" from the enriched s
. The Biological bulletin. Biology; Zoology; Marine biology. COMPARATIVE P'- UPTAKE BY H. ATRA 373 mals were removed from their containers after 6, 9, 12, 25, 36, and 48 hours. The organs of the viscera were removed and separately analyzed for their P'- content. The integument was also subjected to phosphorus analysis. Coelomic fluid was not withdrawn from these sterilized animals. Dehydrating, ashing and counting techniques used for the tissue components of this portion of the study were carried out as ASH WEIGHT INTEGUMENT (grams) Figure 4. The uptake of P'" from the enriched sea-water medium by the integument of un- restricted individual specimens of H. atra. Each point represents a single organism and the uptake rate is measured in counts per minute of P'^ activity per gram of integument per hour of incubation time. The incubation intervals were 6, 12, 24, 30, and 48 hours. Animals with ash weights ranging from to grams were incubated for 6 to 12 hours, while those animals with ash weights between and grams were incubated for 24, 30, and 48 hours. Results The results of the experiments determining the gross removal rate and subse- quent distribution of P^^ to the integument and visceral tissue fractions are shown in Figures 2, 3, and 4. One method for measuring the rate of uptake of P^- from solution is by the disappearance of the isotope with time from the ambient medium. A decrease in isotope activity is illustrated in Figure 2 which shows a reduction in medium activity with increasing length of exposure of intact unrestricted specimens of H. atra. It can be assumed from this curve that the P^- incorporated by the animals is the reverse of the decrease from the solution. The P-'" supplied as phosphoric acid that was removed from solution and incorporated in the integument and combined visceral tissue fractions was mea-. Please note that these images are extracted from scanned page images that may have been digitally enhanced
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectb, booksubjectzoology
