. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 116 R. P. MASON ET AL. I E g1 1-0 pH FIGURE 6. Effect of a mixture of MgCl2 and CaCl2 on the Bohr shift of C. sapidus He in M Tris Maleate at 25°C. Curves are designated by free ion concentration, with MgCl2 given first. Bars show 95% confidence interval. Of the two, Ca+2 is clearly the more important, by a factor approaching six. A factor describing Ca+2 sensitivity (A log P50/A log [Ca+2]) is almost three times greater in C. sap
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 116 R. P. MASON ET AL. I E g1 1-0 pH FIGURE 6. Effect of a mixture of MgCl2 and CaCl2 on the Bohr shift of C. sapidus He in M Tris Maleate at 25°C. Curves are designated by free ion concentration, with MgCl2 given first. Bars show 95% confidence interval. Of the two, Ca+2 is clearly the more important, by a factor approaching six. A factor describing Ca+2 sensitivity (A log P50/A log [Ca+2]) is almost three times greater in C. sapidus (—; Fig. 5) than in C. maenas (—; Truchot, 1975). Indeed, our data (Figures 3 and 5) suggest that, as long as free Ca+2 exceeds 7 meq/1, no other inorganic ion is required to restore HcO2 affinity to the level found in a complete physiological saline. While the effect of Ca+2 is very large and highly specific, a specific Cl~ effect is either absent or so small that it cannot be clearly discerned. The magnitude of the change in He molecular weight following the removal of Ca+2 at physiological pH () (Herskovits et al., 1981) suggests that little, if any, of the Ca+2 effect results from dissociation of the native dodecamer. The absence of a Ca+2 effect on coop- erativity also supports this conclusion. Using chelating agents to remove divalent cations, other investigators (Chantler et al., 1973) have reported an effect of Ca+2 on the cooperativity of portunid crab. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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