. Manual of bacteriology. Bacteriology. METHODS OF ANAEROBIC CULTIVATION. 6l. Fig. -r\o\'}'s anaerobic jar. minutes before closing the coclc in the top of the jar. In effect- ing displacement of the air it is necessary to observe to adjust the cock so that the gas enters directly into the jar from above and finds its way out through the combined rub- ber and glass tubing adjustment from below. In the alternative method, use is made of an ordinary chemical desic- cating jar of suitable size, 6 inches in diameter, in the lower compartment of which is placed about 150 of a i per cent sodium
. Manual of bacteriology. Bacteriology. METHODS OF ANAEROBIC CULTIVATION. 6l. Fig. -r\o\'}'s anaerobic jar. minutes before closing the coclc in the top of the jar. In effect- ing displacement of the air it is necessary to observe to adjust the cock so that the gas enters directly into the jar from above and finds its way out through the combined rub- ber and glass tubing adjustment from below. In the alternative method, use is made of an ordinary chemical desic- cating jar of suitable size, 6 inches in diameter, in the lower compartment of which is placed about 150 of a i per cent sodium hydrate solution in which is dissolved 10 grammes of pyrogallic acid. Into the upper portion of the jar are placed the Petri's dish cultures, and at once the cover of the vessel, pre- viousl}^ smeared with vaseline on its contact surface, is firmly affixed. These vessels are then placed for forty-eight hours in the thermostat before examining. (c) By Bulloch's Apparatus for Anaerobic Culture. — This can be recommended for plating out mixtures containing anaerobes, and for obtaining growths (especially surface growths) of the latter. It consists (Fig. 26) of a glass plate as base on which a bell-jar can be firmly luted down with unguentum resinas. In the upper part of the bell-jar are two apertures furnished with ground stoppers, and through each of the lat- ter passes a glass tube on which is a stopcock. One tube, bent slightly just after passing through the stopper, extends nearly to the bottom of the chamber ; the other terminates imme- diately below the stopper. In using the apparatus there is set on the base- plate a shallow dish, of slightly less diameter than that of the bell- jar, and having a little heap of from two to four grammes of dry pyrogallic acid placed in it towards one side. Culture plates made in the usual way can be stacked on a frame of glass rods. Please note that these images are extracted from scanned page images that may have been digitally enhanced f
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbacteri, bookyear1903
