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. The chemical activities of bacteria. Bacteria. 102 growth: synthesis of bacterial protoplasm cells grown in medium A, rich in pyridoxin; cells grown in medium B, containing just sufficient pyridoxin to allow full growth; and cells grown in medium C, which is deficient in pyridoxin to such an extent that the growth is seriously restricted by the pyridoxin deficiency. If we investigate the activity of the two enzymes, tyrosine decarboxylase (p. 168) and transaminase (pp. 93-4), in the three cultures, we find that both enzymes are fully developed in culture A; in culture B the transaminase syst
. The chemical activities of bacteria. Bacteria. 102 growth: synthesis of bacterial protoplasm cells grown in medium A, rich in pyridoxin; cells grown in medium B, containing just sufficient pyridoxin to allow full growth; and cells grown in medium C, which is deficient in pyridoxin to such an extent that the growth is seriously restricted by the pyridoxin deficiency. If we investigate the activity of the two enzymes, tyrosine decarboxylase (p. 168) and transaminase (pp. 93-4), in the three cultures, we find that both enzymes are fully developed in culture A; in culture B the transaminase system is fully developed, but the. PYRIDOXIN CONTENT OF MEDIUM Fig. 8. Relation of growth of streptococci to pyridoxin content of growth medium. decarboxylase has about 5 per cent of the activity of culture A; in culture C the transaminase activity is considerably less than that of cultures A and B, and no decarboxylase activity is demonstrable. If, however, we take the cells from cultures B and C and re-estimate their activities in the presence of added pyridoxal, we find that both the tyrosine decarboxylase and transaminase activities are restored to normal. This suggests that the organism has synthesised the protein portions of the enzymes in all three cultures, but that the enzymes are inactive in the absence of their prosthetic groups or coenzyme. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Gale, E. F. (Ernest Frederick). New York, Academic Press
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Keywords: ., bookcentury1900, bookcollectionbiodiversity, booksubjectbacteria