. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ZOOXANTHHLLAE IN DIFFERENT HABITATS 355 Samples 1 2 3 4 5 I C°. Figure 9. Examples of srDNA heterogeneity within samples of S\m- biodiniitm C. srDNAs were amplified (using host-excluding PCR primers) from different samples of Symbiodinium C (lanes I-51 and from srDNA clone C" (lane C") and then digested with Dpn II. Taq I. SmMb I. and Hinf I (indicated on the left). Arrows on the right identify DNA fragments that, in digests of srDNA from these samples of zooxanthellae. are additional to the DNA fragments of clone
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ZOOXANTHHLLAE IN DIFFERENT HABITATS 355 Samples 1 2 3 4 5 I C°. Figure 9. Examples of srDNA heterogeneity within samples of S\m- biodiniitm C. srDNAs were amplified (using host-excluding PCR primers) from different samples of Symbiodinium C (lanes I-51 and from srDNA clone C" (lane C") and then digested with Dpn II. Taq I. SmMb I. and Hinf I (indicated on the left). Arrows on the right identify DNA fragments that, in digests of srDNA from these samples of zooxanthellae. are additional to the DNA fragments of clone genotype C". Samples are from Montasrraea annularis (3). M. favvolatu (I. 2. 4) and M. franksi (5). habitats resulted in bleaching of all columns, and effectively reduced zooxanthellar numbers (70% reduction on aver- age). However, neither acute stress (5 days) nor prolonged stress (ffl. 40 days) of zooxanthellae altered the RFLP genotypes that were observed (examples in Fig. 10)—the relative abundance of distinct srDNAs had not changed compared to samples taken prior to transplantation. Discussion Four ta\(i r;/Symbiodinium in the Montastraea annularis complex Previous surveys of zooxanthellar diversity in Monta- straea annularis, M. faveolata, and M. franksi (Rowan and Knowlton, 1995; Rowan et at., 1997) are now shown to be incomplete. In surveys of additional habitats and depths. \se found (i) a fourth taxon of Symbiodinium (E) that was not previously reported in these corals, (ii) differences in the distribution of zooxanthellae at offshore and coastal reefs, and (iii) multiple taxa of zooxanthellae in M. franksi, which previously had been found to contain only Symbiodinium C. Groups A, B, C, and E constitute the known diversity of coral-associated Symhiodin'ntm (Rowan, 1998; this study), and M. annularis, M. faveolata, and M. franksi all associate with at least one member of each of these groups. This is a remarkable amount of taxonomic diversity—at least 12 dist
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
