. Chemical embryology. Embryology. SECT. 9] PROTEIN METABOLISM 1069 that the non-protein nitrogen increases per cent, wet weight in the white, but for the yolk Tomita's rising curve stands in contrast with Aggazzotti's falling one. A private communication from Wright states that recently results have been obtained confirming Aggazzotti on this point (see Fig. 301). Free amino-acids have been found by Fiske & Boyden in the allantoic liquid of the chick, to the extent of 13 mgm. per cent, on the 5th and 7 mgm. per cent, on the loth day of incuba- tion. This is lower than the amino nitrogen o
. Chemical embryology. Embryology. SECT. 9] PROTEIN METABOLISM 1069 that the non-protein nitrogen increases per cent, wet weight in the white, but for the yolk Tomita's rising curve stands in contrast with Aggazzotti's falling one. A private communication from Wright states that recently results have been obtained confirming Aggazzotti on this point (see Fig. 301). Free amino-acids have been found by Fiske & Boyden in the allantoic liquid of the chick, to the extent of 13 mgm. per cent, on the 5th and 7 mgm. per cent, on the loth day of incuba- tion. This is lower than the amino nitrogen of the embryonic blood which Vladimirov & Schmidtt found to vary somewhat erratically between the 13th day and hatching around an average of 54-2 mgm. AggazzoCti. Days -ȉ 5 Fig. 304. per cent. After hatching it has an average value of 52-7 mgm. per cent. Such high values are due to the nucleated erythrocytes. Certain Japanese workers filled an obvious gap in the data which have so far been reviewed by estimating the total nitrogen of the entire egg throughout, and the whole of the free or combined amino- acid nitrogen. The figures of Sakuragi and of Idzumi are shown in Table 135. As Liebermann had originally thought probable, and as Tangl & von Mituch had later made very likely, there is no change at all in the total nitrogen of the whole egg. Not more than an infinitesimal quantity of this element can escape, in agreement with Krogh's findings discussed in the Section on respira- tion. Sakuragi, as the table shows, estimated the nitrogen in the proteins coagulable with acetic acid, on the one hand, and the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Needham, Joseph, 1900-. New York: The MacMillan Co.
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