. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEUROBIOLOGY: SENSORY PHYSIOLOGY AND NEURAL NETWORKS 257 Reference: Biol. Bull 187: 257-258. (October, 1994) Operational Properties of Voltage-Clamped Electroreceptive Ampullary Organ Excised from Raja Jin Lit (University of Texas Medical Branch at Galveston) and Harvey M. Fishman The extraordinary ability of elasmobranch fishes to respond behaviorally to very weak electric fields (10 nV/cm) (1) is thought to be due to the properties of the electroreceptive organ, the ampulla of Lorenzini. We report here the operational volt
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEUROBIOLOGY: SENSORY PHYSIOLOGY AND NEURAL NETWORKS 257 Reference: Biol. Bull 187: 257-258. (October, 1994) Operational Properties of Voltage-Clamped Electroreceptive Ampullary Organ Excised from Raja Jin Lit (University of Texas Medical Branch at Galveston) and Harvey M. Fishman The extraordinary ability of elasmobranch fishes to respond behaviorally to very weak electric fields (10 nV/cm) (1) is thought to be due to the properties of the electroreceptive organ, the ampulla of Lorenzini. We report here the operational voltage range of an isolated organ based on the afferent nerve activity for small voltage (microvolt) clamps of the ampullary epithelium and the stimulus amplitude range for a linear response of the ampullary epithelium. 2 - 0 H ^ 1 1 1 1 : 1 1 1 1 r~ 120 100 80 60 40 20 0 -20 -40 -60 -80-100-120 Ampullary epithelium voltage (uV). 5 10 50 100 500 "00 Transorgan Voltage Stimulus -fiV (rms) Figure 1. (Top) Transfer characteristic of an isolated ampullary organ from skate. The firing rate of the afferent ner\c itinerating the preparation relative to the resting firing rale is plotted in response to irans-ampullary voltage clamps from 0 ml' holding potential (IfPI To minimise effects An ampullary organ consisting of a 3-cm length of canal with ampulla, innervated by a 5-mm length of afferent nerve, was excised from live, anesthetized skates (Raja erinacea and Raja ocellata). The excised organ preparation was placed in a chamber and solutions similar to those described previously by Clusin and Bennett (2). Voltage control of the organ was implemented with an epithelial voltage-clamp system (3) connected to four chlorided-silver pellet electrodes. To voltage clamp the ampullary epithelium, we placed the tip of a long, tapered, electrolyte-filled pipette near the lumenal surface of the ampulla and used it to input the trans-ampullary voltage (referenced to the basal side) to the clam
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
