. 68,000ã 65,000 63,000 43,000 25,700 ^^m 1 2 3 4 5 å¾ä½å¤ç¿»è¯äº§ç©çSDSèä¸ä¸é èºåè¶çµæ³³åæãå¨ä¸ä¾èµmRNAçç½ç»çº¢ç»èè£è§£æ¶²æ ç»èä½ç³»ä¸-ï¼ ä»å¤æºmRNAå¾å°ä½å¤ç¿»è¯äº§ç©ãç»896SDSèä¸ç¯éèºåè¶çµæ³³åï¼éè¿è§å æ¾å½±åææ¾å°æ§èç½è´¨äº§ç©ã æ³³é1:å·²ç¥ç¸å¯¹åå-è´¨éç[14C]-æ è®°çèç½è´¨ï¼ç£·é ¸åæ¦A (93K)ãçè¡æ¸ ç½èç½ï¼68K)ãåµæ¸ èç½ (43K)åa-è°åä¹³èç½é µåï¼)ãæ³³é2~5ä¾æ¬¡æ¯ä¸åmRNAç¨[3H]-è¯æ°¨é ¸æ è®°çç¿»è¯äº§ç©ï¼æ³³é 2ï¼ç½ç»çº¢ç»èè£è§£æ¶²çå æºmRNA;æ³³é3: æ¥èªå°çé¢é¨é§å¸¦ç»polyA+åçRNA;æ³³é4: Mgä¸ æ³³é3ç¸åçRNA,ç¿»è¯äº§ç©ç»5 ^ç¾æ人åå¼¹æ§èç½è¡æ¸ å ç«æ²æ·ï¼æ³³é5: ä¸æ³³é3ç¸åçRNA, ç¿»è¯äº§ç©ç» A
. 68,000ã 65,000 63,000 43,000 25,700 ^^m 1 2 3 4 5 å¾ä½å¤ç¿»è¯äº§ç©çSDSèä¸ä¸é èºåè¶çµæ³³åæãå¨ä¸ä¾èµmRNAçç½ç»çº¢ç»èè£è§£æ¶²æ ç»èä½ç³»ä¸-ï¼ ä»å¤æºmRNAå¾å°ä½å¤ç¿»è¯äº§ç©ãç»896SDSèä¸ç¯éèºåè¶çµæ³³åï¼éè¿è§å æ¾å½±åææ¾å°æ§èç½è´¨äº§ç©ã æ³³é1:å·²ç¥ç¸å¯¹åå-è´¨éç[14C]-æ è®°çèç½è´¨ï¼ç£·é ¸åæ¦A (93K)ãçè¡æ¸ ç½èç½ï¼68K)ãåµæ¸ èç½ (43K)åa-è°åä¹³èç½é µåï¼)ãæ³³é2~5ä¾æ¬¡æ¯ä¸åmRNAç¨[3H]-è¯æ°¨é ¸æ è®°çç¿»è¯äº§ç©ï¼æ³³é 2ï¼ç½ç»çº¢ç»èè£è§£æ¶²çå æºmRNA;æ³³é3: æ¥èªå°çé¢é¨é§å¸¦ç»polyA+åçRNA;æ³³é4: Mgä¸ æ³³é3ç¸åçRNA,ç¿»è¯äº§ç©ç»5 ^ç¾æ人åå¼¹æ§èç½è¡æ¸ å ç«æ²æ·ï¼æ³³é5: ä¸æ³³é3ç¸åçRNA, ç¿»è¯äº§ç©ç» A260 nmå¾®ç²ä½èè¿è¡å ±ç¿»è¯å å·¥ã 2.æ æ ææä½å¤ç¿»è¯ç»åååº-70X:ä¿å-ãè£è§£æ¶²ãå¾®ç²ä½èå[35S]-æ è®°çæ°¨åºé ¸å¯¹ 温度ç¹å«ææï¼åºä»¥éå½ä½ç§¯åè£ ä¿å-äº-70t:并尽éåå°å»èè¿ç¨ï¼æº¶æ¶²å¨è´®å-åç¨ å¹²å°æ液氮快éå·å»ã 1) å¶é ¸æº¶æ¶²ï¼lmg/mlå¶é ¸ï¼ mg/mlç»´çç´ B12ï¼ (w/v) NaCK ;ç¨ æ»¤èè¿æ»¤ï¼åè£ ä¿å-äº-20t:ã 2) è¯è¼æº¶æ¶²ï¼ (w/v)è¯è¼ï¼ % (w/v)ç¢³é ¸æ°¢é ï¼ç¨NaOHè°pHå¼è³ ãæä¸æ¬¡ä½¿ç¨éåè£ ä¿å-äº-20tï¼ä¿å-æä¸è¶ è¿ä¸å¨ãèååæªç¨å®çæº¶æ¶²åº ä¸¢å¼ãè¼çé解ä¼ä½¿æº¶æ¶²ç稻èè²å æ·±ã 3) çççæ°´ï¼ mol/L NaCl, mmol/L MgCl2, 5 mmol/L KC1ã 4t)ä¿å-ã 4) 1 mmol/Læ°¯é«éè¡çº¢ç´ ã 5) mol/L CaCl2ã 6) 7500 U/m
Size: 4635px × 1078px
Photo credit: © The Bookworm Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcollectionbiodiversity, bookcontributorli, booksubjectbotany
