. Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . Fig. I. Chondroitin sulphuric acid. Control sample. Fig. 2. Chondroitin sulphuric acid-trypaflavin complex. The reticular structure characteristic of trypaflavin complexes can be seen very well. when mixed with the dye. The dyes always brought about precipitation. The consistency of the precipi- tates was different with every compound; the most compact precipitate was obtained with rivanol. The results show that the change in structure is, in principle, characteristic for the dye, but in addi- tion the chemical c
. Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . Fig. I. Chondroitin sulphuric acid. Control sample. Fig. 2. Chondroitin sulphuric acid-trypaflavin complex. The reticular structure characteristic of trypaflavin complexes can be seen very well. when mixed with the dye. The dyes always brought about precipitation. The consistency of the precipi- tates was different with every compound; the most compact precipitate was obtained with rivanol. The results show that the change in structure is, in principle, characteristic for the dye, but in addi- tion the chemical composition of the substrate is of importance. These conclusions were supported by our polarized light analysis. We think that the structure which develops is due to a coupling of the macromolecules through the dye molecules. This is supported by the following observation: we solved the newly developed structure by the known ditTusion method with NaOH and ethyl alcohol respectively. On the elimination of either of the components the newly developed structure breaks up. The coupling depends on the steric behaviour both of the polyelectrolyte and the dye molecule. It is obvious that the development of this structure depends on the side groups of the acridine, chiefly of its NH., groups. References 1. DoGSON, K. S., Rose, F. A., and Spencer, B., Biochein. J. 60, 346 (1955). 2. RoMHANYi, G., Congress of the Hungarian Pathological Soc, Debrecen, 1955.
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