A laboratory manual of physiological and pathological chemistryFor students in medicine . with acetic acid evaporateto about 20 cc, at first over a free flame and then on thewater-bath. Mix this with the same volume of absolutealcohol, transfer the mixture to a 100-cc. measuring-flask,rinsing with absolute alcohol, and finally fill the flask up to EXAMINATION OF THE URINE. 103 the mark with tlie same Hquid. Let stand till the next day,filter through a dry filter, and add to the filtrate about twentydrops of an alcoholic solution of zinc chloride. After stand-ing for one or two days crystals of
A laboratory manual of physiological and pathological chemistryFor students in medicine . with acetic acid evaporateto about 20 cc, at first over a free flame and then on thewater-bath. Mix this with the same volume of absolutealcohol, transfer the mixture to a 100-cc. measuring-flask,rinsing with absolute alcohol, and finally fill the flask up to EXAMINATION OF THE URINE. 103 the mark with tlie same Hquid. Let stand till the next day,filter through a dry filter, and add to the filtrate about twentydrops of an alcoholic solution of zinc chloride. After stand-ing for one or two days crystals of creatinine zinc chloride,(C4H-XjO)2ZnCl2, ^^ill be deposited. Examine these underthe microscope. Filter, and wash with alcohol. For identification Weyls reaction may be ased: Grindthe creatinine zinc chloride to a fine powder, boil a smallquantity of this with water in a test-tube, let cool and to the filtrate a few drops of a very dilute solution ofsodium nitroprusside (freshly made) and then some causticsoda solution: deep-red color, which quickly fades to Fig. 9.—Creatinine ZLac Chloride from Urine. The Weyl reaction may also be used directly with theurine. As the urine often contains acetone in considerablequantity and this gives a very similar test, it is advisablebefore trying the reaction to boil the urine for a few minutes,thus driving off the acetone and then cool. Jaffes reaction with picric acid may also be used directlywith the urine: Add to the urine some aqueous picric acidsolution and then a few drops of caustic soda: deep-red color. 104 PHYSIOLOGICAL AND PATHOLOGICAL CHEMISTRY. VI. Detection of Oxalic Acid. Evaporate 500 cc. of unfiltered urine over a small freeflame to about 150 cc. After cooling add 20 cc. of hydro-chloric acid, transfer to a separating-funnel and shake withabout an equal volume of a mixture of alcohol and ether (9volumes of ether, 1 volume of absolute alcohol), carefullyseparate the ether extract and filter it th
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